三嵌段骨基质材料的体外生物学性能评价  

Biocompatibility of Tri-block Bone-matrix Material in vitro

作　　者：郝杰[1] 郑启新[1] 郭晓东[1] 赵明[1] 宋玉林[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《华中科技大学学报（医学版）》2006年第2期247-249,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30200063;No.30170270)

摘　　要：目的 对自主研制的三嵌段骨基质材料——聚丙交酯／乙交酯／天冬氨酸聚乙二醇（PLGA-[ASP—PEG]）的体外生物相容性进行评价。方法 选用建系的L929成纤维细胞进行细胞毒性试验、溶血试验和热原试验；并以PLGA作为对照，计算细胞黏附率。结果 PLGA—EASP-PEG]的毒性评级为0～Ⅰ级；细胞在其表面生长旺盛、形态良好；溶血率为3．08%无热原反应。PLGA-[-ASP—PEG]的细胞黏附率高于PI．GA（P〈0．05）。结论 PLGA-[ASP—PEG]具有良好的生物相容性。Objective To evaluate the blocompatibility of poly（lactic acid/glycolic acid/asparagic acid-co- polyethylene glycol） （PLGA-[ASP-PEG-]） tri-block copolymer in vitro. Methods L929 fibroblasts were used. The cytotoxicity, hemolysis and pyrogen were tested. Compared with PLGA, the adhesiveness rate was calculated. Results The toxicity gradation of the materials was 0- Ⅰ ; L929 fibroblasts had good cell morphology and proliferated rapidly on the surface of the material; hemolysis ratio was 3.08 % there was no pyrogen reaction. The adhesiveness of PLGA-[-ASP-PEG] was better than that of PLGA （P〈 0.05）. Conclusion The PLGA-EASP-PEG3 has good biocompatibility.

关 键 词：组织工程 生物材料 聚乳酸 生物相容性 

分 类 号：R687.3[医药卫生—骨科学]