海嘧啶对细胞膜功能及细胞凋亡影响的研究  

Effect of Haimiding on Cellular Membrane Function and Apoptosis

作　　者：何立巍[1] 季宇彬[2] 孙东东[1] 

机构地区：[1]南京中医药大学,南京210046 [2]哈尔滨商业大学,哈尔滨150076

出　　处：《天津中医药》2006年第2期149-153,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30271598;30300284);黑龙江省自然科学基金重点项目(ZJY0304);黑龙江省教育厅重大项目基金资助(9551Z006);信息产业部项目(01XK230002);黑龙江省普通高等学校骨干教师创新能力资助计划资助(2001015)。

摘　　要：[目的]研究海嘧啶对细胞膜功能以及肿瘤细胞凋亡的影响,阐明海嘧啶抗肿瘤作用机制。[方法]海嘧啶给予S180、H22荷瘤小鼠,观察其红细胞及肿瘤细胞膜功能变化及肿瘤细胞凋亡的情况。细胞膜功能实验测定红细胞膜免疫黏附肿瘤细胞能力、红细胞膜和肿瘤细胞膜上唾液酸含量、红细胞膜和肿瘤细胞膜的封闭度。细胞凋亡实验用流式细胞仪检测细胞分期和凋亡细胞的比例。[结果]海嘧啶对肿瘤细胞和红细胞膜功能均有影响,主要表现在:使荷瘤小鼠红细胞免疫黏附肿瘤细胞的能力有明显提高;可提高红细胞膜唾液酸含量而降低肿瘤细胞膜唾液酸含量;可提高红细胞膜封闭度的同时降低肿瘤细胞膜的封闭度。海嘧啶具有一定程度上的诱发细胞凋亡的作用,不同剂量海嘧啶对细胞周期有不同的影响,小剂量海嘧啶使肿瘤细胞主要集中在G0-G1期,随着剂量的加大,停滞于G2-M期的肿瘤细胞增多。[结论]海嘧啶抗肿瘤作用机制存在多种途径,体现在提高红细胞免疫功能、降低肿瘤细胞膜功能和具有一定程度的诱发肿瘤细胞凋亡的作用。[Objective] To study the effects of Haimiding on the cellular membrane function and tumor cell apoptosis and explain its mechanism of anti-tumor. [Methods] Haimiding was administered to S180, H22, tumor-bearing mice to observe the function of cell membrane of red cell and tumor cell and apoptosis of tumor cells. The determination of the function involved the immunologic adhesive capability of red cells to tumor cells, sialic acid content on red cell membranes and tumor-cell membranes, and the sealing ability of red cell membranes and tumor cell membranes. The apoptosis was measured with Flow cytometry was used to detect the life cycle of cell and the proportion of apoptosis. [Results] Haimiding had effect on tumor cell and red cell membranes functions. They mainly appeared at the obvious increasing of the immunologic adhesive ability of red cells to tumor cells in tumor-bearing mice, increasing of the sialic acid content on red cell membranes and decreasing of it on the tumor cell membranes. Haimiding could also increase the sealing ability of red cell membranes and decrease it of tumor cell membranes meanwhile. Haimiding had some effect on inducing apoptosis and different dose had different influence on cell life cycles. Small dose made tumor cells locaterd mainly at the period of G0-G1. When the dose increased, the number of tumor cells stagnating in the period of G2-M increased. [Conclusions] Haimiding had action of anti-tumor in many ways. It appeared mostly at increasing of the cellular immune functions of red cells, decreasing of the functions of cellular membranes of tumor cells and it had effect on inducing apoptosis in a certain degree.

关 键 词：海嘧啶 红细胞免疫 细胞膜 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]