高效液相色谱法测定人参皂苷Rg1与黄芩苷在细胞培养基中的含量变化  

Determination of Contents of Ginsenosides Rg1 and Baicalin in Cell Culture Medium with HPLC

作　　者：李越[1] 杜嵘[1] 张艳军[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《天津中医药》2006年第2期160-162,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金课题(30472177)。

摘　　要：[目的]研究细胞培养基中人参皂苷Rg1和黄芩苷稳定性问题。[方法]采用高效液相色谱法测定细胞培养基中人参皂苷Rg1和黄芩苷的含量。[结果]人参皂苷Rg1和黄芩苷的线性范围均为20～400ng,方法回收率分别为96.8%和97.7%。[结论]该法简便、灵敏、特异,适用于细胞培养基中人参皂苷Rg1和黄芩苷的测定。[Objective] To study the stability of ginsenosides Rg1 and baicalin in culture medium. [Methods] Ginsenosides Rg1 and Baicalin in culture medium were determined with HPLC. [Results] The linear regressive curves of both substances were in the range of 20-400 ng. The recovery rates of Rg1 and Baicalin were 96.8% and 97.5% respectively. [Condusions] This method is specific, simple, sensitive and suitable for the measurement of Ginsenosides Rg1 and Baicalin in culture medium.

关 键 词：培养基 人参皂苷RG1 黄芩苷 

分 类 号：R285.5[医药卫生—中药学]