人结肠癌组织podoplanin表达与癌组织淋巴管转移关系研究  

The Investigation of the Relation Between the Expression of Podoplanin and the Lymphatical Metastasis in the Human Colonic Carcinoma

作　　者：郑辉[1] 张雅芳[2] 刘保全[2] 

机构地区：[1]黑龙江省齐齐哈尔医学院解剖学教研室 [2]哈尔滨医科大学解剖学教研室

出　　处：《医学研究杂志》2006年第4期8-11,共4页Journal of Medical Research

基　　金：黑龙江省自然科学基金项目(编号D0235)

摘　　要：目的观察结肠癌组织血管、淋巴管内皮细胞podoplanin的表达,为研究癌淋巴管转移的分子机制提供形态学资料。方法根据不同病变阶段的手术病人切除的结肠癌标本进行HE染色和podoplanin的免疫组化染色,观察podoplanin在结肠癌组织中血管和淋巴管的表达情况。结果结肠癌组织血管内皮细胞podoplanin不染色,淋巴管内皮细胞podoplanin阳性表达,同时,随着临床分期的增高和淋巴结转移的发生,podoplanin的阳性表达率增高。结论podoplanin可选择性的表达在结肠癌组织淋巴管内皮上,能明确的区分血管及淋巴管成分,可作为一个淋巴管内皮特有的标志物。同时,癌组织内的淋巴管随着临床分期的增高和淋巴结转移的发生,podoplanin阳性表达率增高,这说明随着肿瘤的进展,结肠癌组织中的淋巴管数量随之增加,可能与结肠癌的淋巴转移有关。Objective To provide the morphology data for the study on the molecular mechanism of tumor metastasis, we observed the expression of podoplanin on the blood vessels and lymphatics in the human colonic carcinoma. Methods We observed the expression of podoplanin on the blood vessels and lymphatics in the colonic carcinoma from the patients of the operation in the defferent stages with the methods of HE staining and podoplanin immunohistochemistry staining. Results Podoplanin has not been stained, but podoplanin was positively expressed on the colonic carcinoma lymphatics. And, The pedoplanin expression positive rate of the colonic carcinoma were increased along with the increase of tumor stages and occurence of lymph nodes metastasis. Conclusion Podoplanin choosed to express in lymphatics in the colonic carcinoma and could correctly distinguish the blood vessels and lymphatic vessels, could be regarded as the specific marker to mark lymphatic vessel. And, The podoplanin expression positive rate of the colonic carcinoma were increased along with the increase of tumor stages and occurenee of lymph nodes metastasis.It indicates, It was correlated with colonic carcinoma lymphatical metastasis that the count of lymphatic vessels were increased in the colonic carcinoma along with the tumor advancing.

关 键 词：结肠癌 淋巴管 PODOPLANIN 

分 类 号：R331.4[医药卫生—人体生理学] R735.370.2[医药卫生—基础医学]