白细胞介素1β刺激脑微血管内皮细胞内15-羟基前列腺素脱氢酶的经时变化  

Changes of 15-PGDH activity in cerebral microvascular endothelial cells stimulated by IL-1β at different time point

作　　者：郭建友[1] 马悦颖[1] 杨元宵 赵保胜[1] 刘洪斌[1] 李兰芳[1] 郭淑英[1] 霍海如[1] 姜廷良[1] 

机构地区：[1]中国中医科学院中药研究所唐氏中药研究中心,北京100700 [2]浙江中医学院,浙江杭州310053

出　　处：《医学研究生学报》2006年第4期330-333,i0013,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金重大项目资助(批准号:90209006)

摘　　要：目的:探讨白细胞介素1β(IL1β)刺激体外培养的大鼠脑微血管内皮细胞(rCMEC)15羟基前列腺素脱氢酶(15PGDH)活性的经时变化。方法:建立rCMEC培养方法,进行Ⅷ因子相关抗原鉴定。待细胞长至融合状态后,以30ng/ml浓度的IL1β分别刺激0.5、1、2、4、8、12、24h,以放射免疫法测定各刺激时间点15PGDH活性。结果:90%以上的培养细胞呈阳性,确定为rCMEC。加入IL1β刺激后2h,细胞内15PGDH活性与未刺激组比较有显著性差异(P<0.05);12h时达到最大值;24h时有所降低,但与未刺激组比较仍具显著性差异(P<0.01)。结论:rCMEC经IL1β刺激后,细胞内15PGDH活性经历一个逐渐升高,到达峰值后缓慢下降的过程,IL1β刺激12h内皮细胞15PGDH活性达峰值。Objective ：To observe the changes of 15-PGDH activity in rats cerebral microvascular endo- thelial cells （rCEMC） stimulated by IL-1β at different time point. Methods： rCMEC were cultured, and identified by immunohistochemistry for von Willebrand factor （ Ⅷ factor, a marker for all endothelial cells） in cytoplasm of the cells. After rCEMC grew to confluency, IL-1β stimulated for 0.5, 1,2, 4, 8, 12 and 24 h respectively. The 15-PGDH activity in rCMEC was measured by isotopic tracing assay. Results：Positive immunostaining for Ⅷ factor was present diffusely in the cytoplasm of 〉 90% rCMEC. Compared with non- IL-1β, the activity of 15-PGDH increased significantly at 2 h after being exposed to 30 ng/ml IL-1β （ P 〈 0.05 ）, and reached the top level at 12 h with （ 119.40 ± 20.36 ） nmol/min · mg-1 protein, then decreased to （91.74 ± 17.86） nmol/min · mg^-1 protein at 24（P 〈0.01 ）. Conclusion： The 15-PGDH activity in rCEMC increased significantly by IL-1β as early as 2 h, reached a maximum production by 12 h of incubation and declined thereafter.

关 键 词：白细胞介素1Β 脑微血管内皮细胞 15-羟基前列腺素脱氢酶 

分 类 号：R392.11[医药卫生—免疫学]