HPLC-UV法测定比格犬血浆中吡非尼酮浓度及其药代动力学  

Determination of pirfenidone in Beagle dog′s plasma by HPLC-UV and study of its pharmacokinetics

作　　者：王永升[1] 赵小平[1] 钟皎[1] 陈燕 柳晓泉[1] 王广基[1] 

机构地区：[1]中国药科大学药物代谢与动力学研究中心,南京210009

出　　处：《中国药科大学学报》2006年第2期146-149,共4页Journal of China Pharmaceutical University

基　　金：国家高技术研究发展计划("八六三"计划)资助项目(NO.2003AA2Z347A);江苏省药物代谢动力学重点实验室资助项目(No.BM2001201)~~

摘　　要：目的：建立测定比格犬血浆中吡非尼酮浓度的方法，用于研究其在比格犬体内的药代动力学。方法：血浆样品用10％高氯酸沉淀蛋白后取上清液直接进样，采用HPLC-UV法测定。色谱柱为汉邦Liehtospher C18柱（250mm×4．6mm ID，5μm），流动相为乙腈．0．2％醋酸水（23：77），流速1．0mL／min，检测波长310nm，内标为水杨酸。结果：线性范围为0．075～76．67班，方法回收率在100．2％～103．3％之间，日内和日间的精密度均小于6％。结论：该方法样品前处理过程简便，灵敏度高，重现性好，符合血浆样品的测定要求，可应用于吡非尼酮比格犬体内的药代动力学研究。Aim：To develop a HPLC-UV method for the determination of pirfenidone in Beagle dog＇s plasma and for the studies of its pharmaeokinetics in Beagle dogs. Methods： Plasma was precipitated with perehlorie acid （10%） after addition of the internal standard salicylic acid, and the upper aliquot was directly injected onto HPLC system. Separation was achieved on a Liehrospher C18 column （250 mm× 4.6 mm ID, 5 μm） with the mobile phase of aeetonitrile-water containing 0.2% acetic acid （23：77） ,at a flow-rate of 1 mL/min. The eluent was detected at 310 nm. ReSults： Linear detection responses for pirfenidone in the plasma were obtained for prifenidone concentration ranging from 0.075 to 76.67μg/mL. The precision data, based on intra- and inter-day variations over 5 days, were lower than 6% and the mean recoveries of prifenidone ranged from 100.2% to 103.3 %. Conclusion：The established HPLC method is simple, sensitive, reliable and applicable for pharmaeokinetie studies of pirfenidone.

关 键 词：吡非尼酮 HPLC 比格犬 药代动力学 

分 类 号：R96[医药卫生—药理学]