汞灯光源芯片毛细管电泳荧光电荷耦合器件检测系统  

Hg-Lamp-Based Fluorescence Charge-Coupled Device Detection in Microchip Capillary Electrophoresis

作　　者：时宝宪[1] 邬丽明[1] 张淑慧[1] 叶丹[1] 黄卫华[1] 程介克[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2006年第2期149-152,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20299034)

摘　　要：以宽谱带高压汞灯为光源的倒置荧光显微镜，配备电荷耦合器件（CCD）及微流控芯片，自组装芯片毛细管电泳荧光电荷耦合器件检测系统。选择不同激发波长（340～540nm）荧光试剂，进行荧光检测，荧光素的检出限（S／N=3）为7．3×10^-9mol／L，荧光素异硫氰酸酯（FITC）的检出限（S／N=3）为1．7×10^-8mol／L，显示了该系统对荧光试剂选择范围宽、灵敏度高的特点。用该系统成功分离了FITC与荧光素、曙红与荧光素两种混合荧光试剂。实验表明，组装的芯片毛细管电泳CCD荧光检测系统是成功的。A detection system in microchip capillary electrophoresis has been developed in this paper, mainly including an inverted fluorescence microscope equipped with a 100 W high pressure mercury lamp and CCD. This system could be applied to the detection of various wavelength fluorescence（340～540 nm）. The detection limit （S/N=3） of 7.3 × 10^-9 mol/L and1.7× 10^-8 mol/L for fluorescein and FITC were obtained, respectively. We have successfully separated FITC-fluorescein and eosin-fluorescein two mixed fluorescent substances. It proved that this fluorescent detection system could be successfully applied to microchip electrophoresis.

关 键 词：芯片毛细管电泳 荧光素 电荷耦合器件 

分 类 号：O657.8[理学—分析化学]