人血清蛋白的共振散射光谱法测定  

RESONANCE SCATTERING SPECTROMETRIC DETERMINATION OF HUMAN SERUM ALBUMIN

作　　者：汪新[1] 刘金水[2] 王伦[2] 

机构地区：[1]池州师范专科学校化学系分析测试研究中心,池州247000 [2]安徽师范大学化材学院,芜湖241000

出　　处：《理化检验（化学分册）》2006年第4期257-259,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：安徽省教育厅自然科学资助研究项目(2004kj296)

摘　　要：在pH7．0的缓冲介质中，吖啶黄（AO）与人血清蛋白（HSA）通过静电引力生成离子缔合物。与单独吖啶黄在相同溶液条件下相比，其共振散射光谱的强度有显著增强。在362nm波长下所测得共振散射光谱峰的强度值与HSA的浓度值在0～1.3mg·L^-1范围内呈线性关系，并求得其检出限为32μg·L^-1。上述反应条件已用于试样中HSA的测定。It was found that in a buffer solution of pH 7. 0, an electrostatic association compound was formed between human serum albumin （HSA） and acridine orange （AO）, leading to a remarkable enhancement of the intensity of resonance scattering spectra measured at 362 nm. Linear relationship between the values of AI （△： I-I0, where I0 is the intensity of the spectral peak without adding HSA） and values of concentration of HSA was obtained in the range of 0 to 1.3 mg · L^-1 of HSA, with a detection limit of 32 μg · L^-1. The above reaction conditions were used in the determination of HSA.

关 键 词：共振散射光谱法 离子缔合物 吖啶黄 人血清蛋白 

分 类 号：O657[理学—分析化学]