紫外分光光度法直接测定AD钙盐中维生素A的含量  

DIRECT UV-SPECTROPHOTOMETRIC DETERMINATION OF VITAMIN A IN AD CALCIUM SALT

作　　者：杨志孝[1] 李桂新[2] 邓文[1] 万惠香[1] 

机构地区：[1]泰山医学院分析实验室 [2]泰山学院,泰安271000

出　　处：《理化检验（化学分册）》2006年第4期267-268,共2页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：AD钙盐中的维生素A是以其乙酸酯的形态加入,而后者不溶于水。根据此性质,将AD钙盐试样用水浸取即可使维生素A与基体组分离,将不溶物分出,溶于乙醇中并定容为10 mL,随即可在此试样乙醇溶液中用UV-分光光度法测定维生素A。在维生素A的吸收峰325 nm波长处,用1 cm石英吸收皿以乙醇作参比液测得其吸光度。用高纯度的维生素A乙醇酯(纯度99.9%)作标准制作标准曲线,得其线性回归方程为cVC=16.899A+0.059,r=0.999 6,方法的回收率在97.7%至100.1%之间。此方法远较国家标准方法和药典方法简单、可行。Vitamin A is added in the AD calcium salt in the form of ethyl ester, which is insoluble in water. Based on this fact, vitamin A was separated from the matrix by extraction with water. The insoluble substance was collected and dissolved in ethanol and diluted to 10 mL quantitatively. Vitamin A in the sample solution was then determined by UV-spectrophotometry. Its absorbance was measured at its absorption maxima of 325 nm, with 1 cm quartz absorption cell using ethanol as reference solution. High purity （99. 9%） ethyl ester of vitamin A was used as standard in the preparation of standard curve, the linearity of which was given by the regression equation cvA = 16. 899 A＋0. 059 （r=0. 999 6）. Results of recovery test obtained were in the range from 97. 7% to 100. 1%. This method was proved to be much simpler than the method given in GB standard and in pharmacopoeia.

关 键 词：UV-分光光度法 维生素A AD钙盐 

分 类 号：O657.32[理学—分析化学]