Cr(Ⅵ)-鲁米诺-过氧化氢-8-羟基喹啉化学发光体系的研究及应用  

STUDY ON THE CHEMILUMINESCENCE REACTION SYSTEM OF Cr(Ⅵ)-LUMINOL-H_2O_2-8-HYDROXYQUINOLINE AND ITS ANALYTICAL APPLICATION

作　　者：李梦耀[1] 张小燕[2] 李绍卿[1] 

机构地区：[1]长安大学环境科学与工程学院,西安710054 [2]西北大学化学工程学院,西安710069

出　　处：《理化检验（化学分册）》2006年第4期278-280,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：基于Cr（Ⅵ）-8-羟基喹啉对鲁米诺-H2O2化学发光体系的催化作用，建立了测定Cr（Ⅵ）的化学发光法。检出限为1．2×10^-3mg·L^-1，线性范围为3．0×10^-3～4．0×10^-2mg·L^-1，回归方程为ICL=3．6＋1．68×10^6C（g·L^-1），相对标准偏差2．8％（n=11）；将该法应用于丙酸钙、动物蛋白粉等生化产品中痕量铬的测定，加标回收率96％～105％，5次测定平均值与苯二碳酰二肼光度法相比较，相对偏差小于5％，并探讨了化学发光反应机理。It was found that in an alkaline medium of KOH, the chemiluminescence （CL） reaction between luminol and H2O2 was catalyzed by the complex formed by Cr（Ⅵ ） with 8-hydroxyquinoline, and that linear relationship between the intensity of CL and the concentration Cr（Ⅵ） ion was kept in the range of 3.0× 10^-3 to 4 .0× 10^-2 mg· L^-1, having its linear regression equation as ICE = 3. 6＋1. 68 ×10^6 C （g · L^-1 ） with correlation coefficient of 0. 999. Detection limit of this method was found to be 1.2 ×10^- 3mg · L^-1 for Cr（Ⅵ ）, under the condition of K=3 and confidential level of 99. 6%. Test of precision was made at the concentration level of 2× 10^-2mg · L^-1 of Cr（Ⅵ） for 11 multiplicate determinations, and the RSD＇s obtained was 2. 8%. In the application of this method to the determination of Cr（Ⅵ ） in samples of calcium propionate and animal protein powder, results obtained were in consistency with the results obtained by photometry. RSD＇s （n= 5） found were less than 3.2%, and recovery tested by the standard addition method gave results in the range of 96% to 105%. A brief discussion on the reaction methanism was also given.

关 键 词：化学发光法 铬(Ⅵ) 8-羟基喹啉 鲁米诺 过氧化氢 

分 类 号：O657.39[理学—分析化学]