郑州儿童医院住院腹泻患儿中星状病毒、杯状病毒的感染分析  

Human astrovirus and calicivirus infection in young children with diarrhea hospitalized at Zhengzhou Children's Hospital

作　　者：孙亚萍[1] 方肇寅[2] 姬振周[3] 章青[2] 谢华萍[2] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150086 [2]中国疾病预防控制中心病毒病所病毒性腹泻室,北京100052 [3]郑州市儿童医院检验科,河南郑州450053

出　　处：《黑龙江医药科学》2006年第2期25-27,共3页Heilongjiang Medicine and Pharmacy

基　　金：世界卫生组织基金资助项目(V27/181/123)

摘　　要：目的：研究郑州儿童医院5岁以下息性腹泻住院患儿中杯状病毒、星状病毒感染的分子流行病学特点。方法：对郑州儿童医院2001-11～2002—10收集的302份腹泻粪便标本中的96例非轮状病毒腹泻标本应用RT—PCR方法进行杯状病毒、星状病毒检测，并对阳性标本通过RT—PCR或测序分析鏊定型别。结果：实验中共检出9份杯状病毒阳性标本、4份星状病毒阳性标本，检出率分别为9．4％（9／96）和4．2％（4／96），其中9份杯状病毒阳性标本中8株属于诺如病毒GII／4型、1株为GII／3型，4株星状病毒阳性标本均为HAstV-1型。结论；杯状病毒、星状病毒也是郑州地区婴幼儿病毒性腹泻的重要病原体，杯状病毒、星状病毒的流行型别分别为诺如病毒GII／4型、HAstV-1型。Objective： To study molecular epidemiological features of calicivirus and astrovirus infection in children under 5 years of age hospitalized for acute diarrhea in the Chidren＇s Hospital, Zhengzhou. Methods： Stool samples were collected from the children with diarrhea between november 2001 and october 2002. of 302 samples, 96 negative for rotaviruses and bacterial pathogens were tested for calicivirus and astrovirus by using a reverse transcription- polymerase chain reaction （RT- PCR）, genotyping of positive samples was performed by type-specific RT-PCR or confirmed by sequence analysis. Results ：of 96 fecal specimens, calicivirus in 9 and astrovirus in 4 samples were detected, with the detection rate of 9.4% and 4.2%, respectively. Four specimens with HAstV （serotypes 1）, eight with NoV （ GII/4 ） and one with NoV （GII/3） were observed, respectively. Conclusion： Calicivirus and astrovirus are important causative agents in young children with virus diarrhea in the Chidren＇s Hospital, Zhengzhou, with HAstv-1 and NoVGII/4 as the predominant type.

关 键 词：杯状病毒 星状病毒 分型引物 RT—PCR 

分 类 号：R373.2[医药卫生—病原生物学] R442.2[医药卫生—基础医学]