基质γ-羧基谷氨酸蛋白和氨基乙酰丙酸脱水酶基因多态性与儿童血铅的关系  

The Association of Gene Polymorphisms of MGP and ALAD with Lead Blood Level in Children

作　　者：张文众[1] 冷曙光[1] 戴宇飞[1] 王雅文[1] 牛勇[1] 阎慧芳[1] 吴宜群[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《环境与职业医学》2006年第2期112-114,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金(编号:39770640)

摘　　要：[目的]探讨基质γ-羧基谷氨酸蛋白(MGP)和氨基乙酰丙酸脱水酶(ALAD)基因多态性在儿童铅中毒发生过程中的作用。[方法]测定了345名6-12岁的中国汉族儿童的血铅水平;采用多聚酶链反应-限制性片段长度多态性法(PCR-RFLP)分析MGP和ALAD基因的多态性。[结果]在ALAD基因未发生变异的情况下,MGP基因变异导致血铅水平降低;携带ALAD1-2、ALAD2-2或MGP TT基因型儿童的血铅水平(2．32μmol/L)高于携带ALAD1-1和MGP CT、MGP CC基因型儿童(2．18μmol/L),差异有统计学意义(P=0．0091)。[结论]MGP和ALAD基因多态性可影响儿童铅中毒的易感性,ALAD1-2、ALAD2-2或MGP TT基因型可能是儿童铅中毒的易感基因型。[ Objective ] To explore the association of polymorphisms of MGP gene and ALAD gene with lead poisoning in children. [ Methods ] 345 children were recruited into this study and the blood lead was measured. The polymorphisms of MGP gene and ALAD gene were analyzed by the methods of PCR-RFLP. [ Results ] In wild type of ALAD gene, mutation type of MGP gene may decrease blood lead level in children. The children with ALADI-2/2-2 or MGP TT genotype compared to children with ALADI-1 and MGP CT/CC genotype, the blood levels were higher（ 2.32 μmol/L.vs.2.18μmol/L, P = 0.0091 ）. [ Conclusion ] The results indicate there is joint effect between polymorphism of MGP gene and ALAD gene and susceptibility to lead poisoning in children.

关 键 词：儿童铅中毒 MGP基因 ALAD基因 单核苷酸多态性 

分 类 号：Q786[生物学—分子生物学]