八氯二丙醚吸入染毒对小鼠脏器组织的氧化损伤作用  

Effect of Octachlorodipropyl Ether Inhalation on Oxidative Damage in Mice

作　　者：曾垂焕[1] 唐萌[1] 熊丽林[1] 王晓娜[1] 夏婷[1] 卢毅[1] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009

出　　处：《环境与职业医学》2006年第2期151-152,163,共3页Journal of Environmental and Occupational Medicine

基　　金：江苏省预防医学基金资助项目(编号:Y200421)铁道部科技基金资助项目(编号:674780030)东南大学科技基金资助项目(编号:XJ019005)

摘　　要：[目的]研究八氯二丙醚(octachlorodipropyl ether)吸入染毒对小鼠肝、肾、脾、肺细胞的氧化损伤作用。[方法]采用静式吸入染毒法,研究不同染毒羰基含量(282、564、1128 mg/m3,连续染毒20 d,每天1h)对小鼠的氧化损伤作用,染毒结束后测定各脏器丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及羰基含量。 [结果] 高浓度(1128 mg/m3)染毒组中,小鼠肝、脾、肺MDA、羰基含量与对照组比差异有显著性(P<0．05);肺 MDA含量、羰基含量随染毒浓度的增加而增加,且呈剂量-反应关系(P<0．05)。高浓度组肝、脾、肺SOD、GSH-Px的活性下降且与对照组比差异有显著性(P<0．05);肾脏细胞各观察指标与对照组的差异均不显著。[结论]八氯二丙醚吸入染毒可引起小鼠肝、脾、肺的氧化损伤,肺脏可能是其主要的靶器官。[ ObJective ] To study the effect of octachlorodipropyl ether （ Sz ）inhalation on oxidative damage of liver, kidney, spleen and lung in mice. [ Methods ] Static inhalation chamber was applied for exposing mice to S2 （ 282, 564, 1128 mg/m^3, lh each day for 20 days ）. Concentration of MDA, SOD, GSH-Px and carbonyl contents in some organs were detected to study oxidative damage of S2. [ Results ] In the high concentration group（ 1128 mg/m^3 ）, MDA and earbonyl contents of liver, spleen and lung were significantly different from those in the control group（ P 〈 0.05 ）; MDA and carbonyl contents of lung increased with concentration of S2（ P 〈 0.05 ）with a dose-response relationship. In the high concentration group SOD and GSH-Px activities were reduced and significantly different to the control group（ P 〈 0.05 ）; There were no significant difference in kidney between the test and control group（ P 〉 0.05 ）for all of the oxidative damage indexes. [ Conclusion ] Inhalation of S2 can cause oxidative damage in liver, spleen and lung in mice, and lung might be the main target.

关 键 词：八氯二丙醚 氧化损伤 羰基含量 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]