应用单光子发射计算机断层扫描观察家兔“风门”穴皮下、皮内及静脉注射核素钼锝的走行通路  

Passageway of molybdenum and technetium injected into subcutaneous part and subcuticular part of Fengmen acupoint and vein scanning with single photon emission computed tomography

作　　者：雷玲[1] 章云海[1] 宋长祥[2] 卢文[3] 李玉[4] 曾因明[5] 李静[1] 孙波[2] 徐志强[1] 耿祝生[1] 张兆剑[1] 刘克芳[1] 

机构地区：[1]连云港市第一人民医院麻醉科,江苏省连云港市222002 [2]连云港市第一人民医院核医学科,江苏省连云港市222002 [3]连云港市第一人民医院针灸科,江苏省连云港市222002 [4]连云港市第一人民医院瘤生物实验室,江苏省连云港市222002 [5]连云港市第一人民医院江苏省麻醉学重点实验室,江苏省徐州市221002

出　　处：《中国临床康复》2006年第15期126-128,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30271260);江苏高校省级重点实验室第三批对外开放课题K2093);江苏省科技厅指导计划课题(BS2002305);连云港市科技局课题(SH0408)~~

摘　　要：目的:应用单光子发射计算机断层扫描观察家兔通过皮下、皮内、静脉3种不同途径注射核素钼锝“风门”穴的皮部腧穴通路,分析腧穴与皮肤感受器的关系。方法:实验于2005-10/11在徐州医学院附属连云港医院肿瘤生物实验室完成。将6只家兔分为皮下注射组,皮内注射组和静脉注射组3组,每组2只,皮下注射组在已确定的家兔胃伤害性刺激的皮肤牵涉区,取双侧“风门”穴,左侧皮内、右侧皮下注射核素钼锝(99TcmO4-,99Tcm)为示踪剂,皮内注射组取双侧″风门″穴,双侧皮内注射99Tcm,静脉注射组经静脉注射99Tcm。各组均应用单光子发射计算机断层扫描成像,分别从兔冠状面和矢状面收集示踪剂图像,示踪剂选择绿色成像,示踪剂集中区呈白色。每次连续收集图象16幅,成像时间为7min,开始每隔15min连续收集图像1次,1h后每隔30min连续收集图像1次,3h后每隔60min连续收集图像1次,6h后每隔120min连续收集图像1次,观察至注射核素后12～14h。结果:①实验家兔6只均成功按设计方案进行了示踪剂的注射,3组实验共获单光子发射计算机断层扫描图像1280幅。②皮下、皮内、静脉3种不同途径注射核素钼锝通过SPECT观察不同的形态学变化:“风门”穴皮内注射的99Tcm沿纵向行走,延长约2～5倍,注射12～14h后仍清晰可见;“风门”穴皮下注射的99Tcm逐渐缩小,8～10h消失;静脉注射的99Tcm,8～10h大部分经肾脏排泄。结论:①家兔皮内注药有两条吸收途径,即血液和神经末梢感受器吸收途径。②皮下注射主要通过血液吸收。③腧穴部位皮内示踪剂沿纵向行走,有可能与皮内神经末梢感受器的逆行吸收有关,与交感感受器关系有可能更密切,皮部腧穴有可能是皮内感受器的密集点或片,皮部经络有可能是皮内感受器密集点的有序排列。AIM： To observe passageway of molybdenum （Mo） and technetium （Tc） injected into subcutaneous part, subcuticular part and vein of Fengmen acupoint scanning with single photon emission computed tomography （SPECT） and analyze the relationship between Shu acupoint and skin receptor. METHODS： The experiment was completed the Biochemical Laboratory of Tumor of Lianyungang Hospital Affiliated to Xuzhou Medical College from October to November 2005. Totally 6 rabbits were divided into subcutaneous injection group, intradermal injection group and vein injection group with 2 in each group. Bilateral Fengmen acupoints were collected in stomach noxious-stimulation skin-involving area of rabbits in subcutaneous injection group, intradermal injection on left side and subcutaneous injection on right side were performed with^99Tc^mO4^- and which were regarded as tracer. Rabbits in intradermal injection group were injected into bilateral Fengrnen acupoints with ^99Tc^m, and also with ^99Tc^min vein injection group. Imaging in three groups was all scanned with SPECT, and image of tracer was collected from coronal plane to sagittal plane, respectively. The image of tracer was green, and concentration of tracer was white. A total of 16 images were collected successively within 7 minutes. Image was collected successively once every 15 minutes at the beginning; 1 hour later, image was collected successively once every 30 minutes; 3 hours later, image was collected successively once every 60 minutes; 6 hours later, image was collected successively once every 120 minutes. All images were observed 12-14 hours after injection of nuclein. RESULTS： ① A total of 6 rabbits entered the injection of tracer, and images scanned with SPECT were 1 280 in total in the three groups, ② Morphological changes of Mo and Tc scanned with SPECT through subcutaneous injection, intradermal injection and vein injection： Intradermal injection with ^99Tc^m at Fengmen acupoint moved longitudinally, delayed 2-5 times, and was observed

关 键 词：体层摄影术 发射型计算机 单光子 有机锝化合物 放射性药物 注射 皮内 经脉 穴 输(五输) 

分 类 号：R814.42[医药卫生—影像医学与核医学]