RT-PCR检测猪传染性胃肠炎病毒的研究  

Detection of Transmissible Gastroenteritis Virus (TGEV) by Reverse Transcription-Polymerase Chain Reaction

作　　者：于天飞[1] 王君伟[1] 胡森[1] 靳淼[1] 

机构地区：[1]东北农业大学动物医学院预防兽医微生物教研室,黑龙江哈尔滨150030

出　　处：《中国动物检疫》2006年第5期21-23,共3页China Animal Health Inspection

基　　金：黑龙江省"九五"攻关课题(G00B032021)

摘　　要：根据GenBank发表的猪传染性胃肠炎病毒(TGEV)纤突蛋白S基因的5′端核酸序列设计了1对引物。该对引物扩增的目的片段长度为690bp。在优化RT-PCR反应条件的基础上,建立了TGEV的RT-PCR检测方法。According to published sequence in GenBank, a pair of oligonucleotide primers appropriate for PCR amplication were selected based on the two conserved regions of the 5＇end of the gene encoding the S protein of TGEV. A reversetranscription-polymerase chain reaction （RT-PCR） assay was developed for detection of TGEV after optimization of the reaction conditions. All of 15 samples were positive detected by RTPCR.

关 键 词：猪传染性胃肠炎病毒 S基因 RT-PCR 

分 类 号：S852.659.6[农业科学—基础兽医学] S662.5[农业科学—兽医学]