己烯雌酚多克隆抗体的制备和鉴定  

Preparation and characterization of polyclonal antibody to Diethylstibestrol

作　　者：古艳丽[1] 郑增忍[2] 刘文字[3] 陈德坤[1] 路平[2] 刘爽[2] 李葳[2] 曲志娜[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]农业部动物检疫所,山东青岛266032 [3]华南农业大学兽医学院,广州510642

出　　处：《中国动物检疫》2006年第5期23-25,共3页China Animal Health Inspection

摘　　要：用混合酸酐法合成的BSA-DES免疫兔子,制备抗DES的特异性抗体,分别用混合酸酐法和碳二亚胺法合成的OVA-DES作为包被原检测抗体;用混合酸酐法合成的OVA-DES和碳二亚胺法合成的OVA-DES免疫小白鼠,制备抗DES的特异性抗体,分别用混合酸酐法合成的BSA-DES和碳二亚胺法合成的BSA-DES作为包被原检测抗体。检测结果表明:三种免疫原免疫动物,都产生了DES的特异性抗体,并建立了间接阻断ELISA法。这为残留DES酶免疫检测试剂盒的制备奠定了基础。To prepare the anti-DES antibody, rabbits were immunized with BSA-DES which was synthesized in mixed anhydride and isobutylchlorofomate, and OVA-DES which were synthesized respectively by mixed anhydride and Isobutylchorofomate and carbodiimide method were used coating antigens to identify antibody in ELISA; Mice were immunized by OVA-DES which respectively were synthesized by mixed anhydride and Isobutylchorofomate and carbodiimide method, and BSA-DES which were synthesized respectively by mixed anhydride and Isobutylchorofomate and carbodiimide method were coating antigens to identify antibody in ELISA; The results show that anti-DES polyclonal antibody were produced by rabbits and mice, and indirect inhibition ELISA method was established. It＇s very important to develop DES residual immunoassay reagent kit.

关 键 词：己烯雌酚 免疫原 包被原 多克隆抗体 

分 类 号：S859.84[农业科学—临床兽医学] Q786[农业科学—兽医学]