survivin与X射线诱导的染色体不稳定性  

Survivin and chromosome instability induced by X-irradiation

作　　者：沈波[1] 鞠桂芝[1] 刘扬[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物重点实验室,长春130021

出　　处：《中华放射医学与防护杂志》2006年第2期129-132,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(30270346)

摘　　要：目的探讨survivin基因在X射线诱导染色体不稳定性中所起的作用。方法用免疫细胞化学的方法检测survivin蛋白在HeLa细胞中的表达水平,并通过转染小片段发卡状RNA干扰HeLa细胞中survivin蛋白表达。转染后细胞接受4GyX射线照射,用流式细胞术检测照后0、4、12和48h的多倍体细胞发生率,并与单纯接受4GyX射线的对照组相比较。结果转染小片段发卡状RNA能够有效抑制HeLa细胞中survivin蛋白表达,转染48h抑制率达到32.16%。细胞接受4GyX射线照射后,多倍体细胞所占比例在48h时高于对照组(P<0.001)。而抑制survivin蛋白表达后,多倍体细胞所占比例于12和48h明显升高,大约是对照组的2倍以上。结论X射线照射可以诱导HeLa细胞发生染色体不稳定性。干扰survivin表达可以加强该效应,因而survivin在维持染色体稳定性方面发挥着重要作用。Objective To explore the biological effect of survivin on chromosome instability induced by X-ray irradiation. Methods lmmunocytochemistry was used to detect the expression of survivin in HeLa cells. Carrier pSUPER-SVV was transfected into HeLa cells to interfere the expression of survivin. Flow cytometry assay was applied to detect the occurrence of polyploid at 0 h, 4 h, 12 h, and 48 h after the HeLa cells transfected with pSUPER-SVV and irradiated with 4 Gy X-rays irradiation, and compared with the group irradiated with 4 Gy X-rays but no transfection. Results The expression of survivin was down-regulated by transfecting with small hair RNA, its depression rate was estimated to be about 32.16% at 48 h after transfeetion. The occurrence of polyploid gaint cells was higher in the 4 Gy X-ray irradiated group at 48 h after the irradiation than the control groups （P 〈 0.001）. Being expression of survivin interfered, the occurrence at 12 h or 48 h after irradiation, however, was about two times higher than that in the control group. Conclusion X-ray irradiation can induce chromosome instability in HeLa cells and the effect could be enhanced by interfering the expression of surviving. It was suggested that survivin plays an important role in maintaining the stability of chromosome.

关 键 词：染色体不稳定性 SURVIVIN基因 RNA干扰 X射线诱导 

分 类 号：R818.02[医药卫生—放射医学]