中子辐射后骨髓造血功能损伤以及IL-11受体的表达变化  

The expression and significance of IL-11 receptor in bone marrow cells of mice with neutron irradiation

作　　者：韩瑞刚[1] 彭瑞云[1] 高亚兵[1] 王瑞娟[1] 付凯飞[1] 马俊杰[1] 陈建魁[2] 胡文华[1] 董波[1] 王晓民[1] 

机构地区：[1]北京放射医学研究所,100850 [2]解放军第三○七医院

出　　处：《中华放射医学与防护杂志》2006年第2期133-136,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：全军"十五"指令性课题资助项目(01L019);国家自然科学基金资助项目(30370438)

摘　　要：目的探讨IL11受体在中子照射骨髓造血细胞损伤后的变化规律及其意义。方法100只雄性BALB c小鼠经2.5和4.0Gy中子全身照射,于照射后6h,1、2、3、5、7、14和28d分批活杀,应用光镜和电镜观察骨髓病理变化;流式细胞术和DNA凝胶电泳检测骨髓细胞凋亡;免疫组化和图像分析定量检测IL11Rα及gp130的表达。结果2.5Gy中子照射后3d内,小鼠骨髓造血细胞见凋亡与坏死,数量进行性减少;至照射5d后逐渐恢复,28d恢复至正常。4.0Gy中子照射,骨髓损伤更严重,未见恢复。2.5Gy中子照射后1d内,IL11Rα在造血细胞膜及胞浆略有增加,2～7d减少,14d后逐渐恢复;而gp130于照射后7d内在造血细胞膜及胞浆减少,14d后逐渐恢复。4.0Gy照射后2d内,IL11Rα和gp130均进行性减少,较2.5Gy减少更为明显。结论IL11Rα及gp130表达在中子照射后骨髓损伤期减少,而于恢复期逐渐恢复,参与了骨髓辐射损伤后造血功能重建的病理生理过程。Objective To study the change and its significance of IL-11 receptor of bone marrow cells of mice irradiated with neutron. Methods 100 BALB/c mice were whole-body irradiated with neutron of 2.5 and 4.0 Gy, then killed at 6 hours and 1, 2, 3, 5, 7, 14 and 28 days after the irradiation. Pathologic changes of bone marrow were observed using optical microscope and electron microscope. The apoptosis and necrosis of hematopoietic cells were evaluated by flow cytometry and DNA gel electrophoresis. The expressions of IL-11R and gp130 were measured by immunocytochemistry and image analysis. Results Apoptosis and necrosis were occurred in hematopoietic cells, and the number of the cells was progressively decreased within 3 days after neutron irradiation of 2.5 Gy, then began to recover at 5 days and completely recovered at 28 days after the irradiation. Irradiation with neutron of 4.0 Gy induced severe injury in bone marrow, and the damaged to bone marrow was hardly recovered. IL-11Rα protein slightly increased in the cell membrane and cytoplasm of hematopoietic cells at 1 day after irradiation with 2.5 Gy neutrons, then progressively decreased through 2 to 7 days, and began to gradually recover starting from the 14th days after the irradiation. Gp130 protein, however, was progressively decreased within 7 days and then gradually increased after 14 days of irradiation. With neutron irradiation of 4.0 Gy, both IL-11 Rot and gp130 protein decreased more significantly within 2 days than the group irradiated with 2.5 Gy neutrons. Conclusion IL-11Rα and gp130 protein were observed to be decreased in injured hematopoietic cells and then increased during the recovery time, which suggested the expressions of both proteins may play an important role in the reconstruction of injuring hematopoietic cells after irradiation.

关 键 词：中子 骨髓 病理 IL-11Rα GP130 中子辐射 骨髓造血功能损伤 

分 类 号：R818.74[医药卫生—放射医学]