高功率微波辐照对大鼠血睾屏障通透性的影响  

Effect of blood-testis barrier after exposure to high power microwave (HPM) in rats

作　　者：王爽[1] 黄晓峰[1] 王春梅[1] 杨家骥[1] 李珍[1] 黄小军[1] 于华[1] 

机构地区：[1]第四军医大学基础部电子显微镜中心,西安710032

出　　处：《中华放射医学与防护杂志》2006年第2期186-188,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家863课题基金资助项目(2002AA832033)

摘　　要：目的探讨高功率微波(HPM)辐照大鼠睾丸对血睾屏障的影响。方法雄性SD大鼠35只,随机分为7组,每组5只。6组为辐照组,1组为对照组。于辐照后2、6、12、48、96h和8d活杀大鼠后取双侧睾丸组织各约1mm3,镧醛固定液固定后制备电镜标本,在透射电镜下进行观察。结果对照组可见硝酸镧主要沉积在曲细精管基膜部和精原细胞周围,未越过紧密连接;辐照后2～6h电镜下所见与对照组大致相同;辐照后12～96h,从基底部到管腔面的各层生精细胞之间、支持细胞内都有大量的镧颗粒沉积;辐照后8d,仍可见细胞间隙增大,部分区域靠近精子表面的镧颗粒沉积甚至比前面时间点的更为严重。结论高功率微波辐照能够改变细胞的通透性,破坏血睾屏障的保护作用。Objective To study the effect of blood-testis barrier after exposure to high power microwave （HPM） in rats. Methods Thirty-five SD rats were divided into two groups： the experimental group treated with HPM and the control group. At different time points （2 hours, 6 hours, 12 hours, 48 hours, 96 hours, 8 days）. After exposure to HPM, the rats were killed to take 1 mm^3 tissue on the left and right testis. The specimens were fixed in lanthanum-glutaraldehyde solution. Preparation and routine transmission electron microscopy （TEM） were performed respectively. Results The lanthanum precipitation was found in basal membrane of the seminiferous tubule and around and spermatogonium in control group, they didn＇t across the tight junction. At 2-6 hours after exposure to HPM, same results as control group＇s were found. At 12-96 hours after exposure to HPM, the large number of lanthanum precipitations were also found from the basal part to spermatogenic cell and Sertoli cell. On day 8 after exposure, the intercellular space were enlarged, the lanthanum was deposited strongly in partial area. Conclusion Exposure to HPM might change the cellular permeability of cell membrane and destruct the protection of the blood-testis barrier.

关 键 词：高功率微波 曲细精管 血睾屏障 透射电镜 大鼠 

分 类 号：R594.8[医药卫生—内科学]