三七皂苷Rg_1对大鼠脑缺血再灌注损伤海马部位保护作用机制的研究  

Study on Protective Mechanism of Notogisenoside-Rg_1 in Hippocampi of Focal Cerebral Ischemia Reperfusion Injury in Rats

作　　者：吴兰鸥[1] 詹合琴[1] 闫俊岭[1] 蔡文锋[1] 吴进平[1] 杨克红 

机构地区：[1]昆明医学院药理学教研室,昆明650031

出　　处：《天然产物研究与开发》2006年第2期219-224,245,共7页Natural Product Research and Development

基　　金：云南省自然科学基金项目(2002C0051m)

摘　　要：本文探讨三七皂苷Rg1对局灶性脑缺血再灌注损伤大鼠海马部位的脑源性神经营养因子(brain-derivedneurotrophic factor,BDNF)阳性蛋白的含量和阳性神经元数目是否具有上调作用。实验结果表明三七皂苷Rg1高、中、低剂量组和阳性对照组均能明显改善脑缺血的神经缺失症状,并能上调大鼠脑缺血再灌注损伤海马部位的BDNF阳性蛋白的含量和阳性神经元数量(P<0.05);与阳性对照组(尼莫地平1 mg/kg)相比,用药7 d时,Rg1中剂量组(100 mg/kg)在改善脑缺血的神经缺失症状以及上调大鼠脑缺血再灌注损伤海马部位的BDNF阳性蛋白的含量和阳性神经元数量方面,作用上强于尼莫地平(P<0.05)。三七皂苷Rg1能上调BDNF阳性蛋白的表达,通过BDNF对脑缺血再灌注神经元损伤所起的保护作用,从而发挥其对脑缺血的治疗作用,这可能是三七皂苷Rg1对脑缺血保护作用的机制之一。To study the effects of notogisenoside-Rgl on brain-derived neurotrophic factor（BDNF） protein in hippocampi of fccal cerebral ischemia repeffusion injury, as well as to investigate whether notogisenoside-Rg1 can up-regulate the protein content of BDNF of the positive neurons and the amount of the positive neurons. 60 Adtdt male SD rats, weighing 280 - 320 g, were randomly divided into model group,high dose group,middle dose group,low dose group, and the positive control group： Comparing with the model group,all notogisenuside-Rg1 treated groups and the positive control group can obviously improve some nervous deficit symptoms in the rat .They also can increase the protein content of BDNF of the positive neurons and the amount of the positive neurons in the hippocampi of focal cerebral ischemia repeffusion injury in rat（ P 〈 0.05）. Comparing with the positive control group,in middle dose treated group （100 mg/kg） with 7 d,the effect of notogisenoside-Rg1 was superior to the nimodipine（1 mg/ kg） on flint improve some nervous deficit symptoms,as well as increase the protein content of BDNF of the positive neurons and the amount of the positive neurous. Notogisenoside-Rg1 could up-regulate the expression of BDNT and increase the amount of positive nettrous in hippocampi. It treated cerebral ischemia by the protection of BDNF on neurons injury in the fccal cerebral ischemia-reperfusion,which can be one of the protective mechanism of gisenoside-Rg1 on focal cerebral ischemia repeffusion injury.

关 键 词：三七皂苷RG1 脑缺血 再灌注损伤 BDNF 上调作用 大鼠 

分 类 号：R286.1[医药卫生—中药学]