薯蓣皂甙元ELISA定量分析方法的建立I—薯蓣皂甙元全抗原的合成  

Synthesis of Diosgenin-BSA and Diosgenin-OVA Conjugates

作　　者：杨红艳[1] 袁丽红[1] 吴红利[1] 于潇[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《天然产物研究与开发》2006年第2期254-256,共3页Natural Product Research and Development

摘　　要：建立薯蓣皂甙元的ELISA定量分析方法必须先合成薯蓣皂甙元的全抗原。试验利用薯蓣皂甙元3位上的-OH,在DMAP的催化下,薯蓣皂甙元与丁二酸酐反应,生成薯蓣皂甙元丁二酸单酯,用MSI、R1、H NMR1、3CNMR等方法对产物结构进行了表征。用混合酸酐法制备薯蓣皂甙元与牛血清白蛋白的结合物(DG-HS-BSA),碳二亚胺法制备薯蓣皂甙元与卵清蛋白的结合物(DG-HS-OVA),经TNBS测定,每分子结合物连接的薯蓣皂甙元分子数分别为28.0和8.8。An enzyme-linked immunosorbent assay（ELISA）to detect and quantify diosgenin in cell or tissue cultures is being developed.In the presence of succinic anhydride and catalyst DMAP, diosgenin was derivatized into diosgenin bemisuccinate, with reactive carboxyl group. The product was characterized by MS, IR,^1HNMR and 13 C-NMR spectral data. Diosgenin hemisuccinate was coupled with BSA by mixed anhydride method and ovalbumin by method of water-soluble carbedlimide. The number of diosgenin residues per molecule of the conjugates was 28.0 and 8.8, respectively. The suecessfid preparation of diosgenin-protein conjugates made it possible to quantify diosgenin by ELISA.

关 键 词：薯蓣皂甙元二酸单酯 薯蓣皂甙元 牛血清白蛋白 卵清蛋白 

分 类 号：Q946.91[生物学—植物学] O652.1[理学—分析化学]