氧化苦参碱的提取分离及对小鼠的毒性研究  

Study on Separaing and Toxicity to Mus musculus of Oxymatrine

作　　者：张宏利[1] 贺春玲[2] 杨清娥[1] 杨学军[1] 韩崇选[1] 

机构地区：[1]西北农林科技大学林学院,杨凌712100 [2]河南科技大学林业职业学院,洛阳471002

出　　处：《天然产物研究与开发》2006年第2期279-281,272,共4页Natural Product Research and Development

基　　金：国家"十五"科技攻关项目(2001BA509B07-2-02);国家林业局重点项目(2002-20)

摘　　要：采用酸性乙醇提取、硅胶柱层析分离、乙醚分级沉淀、丙酮重结晶的方法从苦参中分离到氧化苦参碱单体。对小鼠的毒性测定表明,试鼠的死亡主要集中在48 h内,48 h后无试鼠的死亡现象。小鼠对氧化苦参碱的耐受量大于50 mg/kg,小于20 mg/kg,致死中量LD50为85.95 mg/kg,回归方程Y=-3.2664+4.2737X,LD50标准误差s=8.77。氧化苦参碱毒性较低,致死中量适宜,对试鼠有较好的适口性,可以作为杀鼠剂使用。Oxymatrine was obtained from Sophoraflavescens by means of extracting with 80% acidity alcohol, separation by silica gel, precipitation by ether. The toxicity test showed most test rats died within 48 h. No mere rat died after 48 h. Test rats＇ endurance of drug mete for oxymatrine is between 50 mg/kg and 200 mg/kg, with LD50 = 85.9543, The regressive equation is： Y = - 3.2664 ＋ 4.2737X, with s = 8.77. Toxieily is low, LD50 is suitable of oxymatrine and edibality is good for test rat. It can be used for rodentieide.

关 键 词：氧化苦参碱 提取分离 小鼠 毒性 

分 类 号：S764.5[农业科学—森林保护学]