人骨髓间充质干细胞诱导分化为内皮细胞的实验研究  

Experimental study of incubating HBMCs into ECs for oriented differentiation

作　　者：徐成阳[1] 冯德广[1] 杜英[2] 赵根尚[1] 蒋素娟[1] 余海滨[1] 于海明[1] 黄玉敏[2] 

机构地区：[1]郑州大学第二附属医院心血管外科,郑州450014 [2]郑州大学医学院微免教研室

出　　处：《中原医刊》2006年第8期22-23,共2页Central Plains Medical Journal

摘　　要：目的探讨将人骨髓间充质干细胞(HBMCs)诱导为内皮细胞(ECs)的可行性。方法将HBMCs分离、培养后,加入诱导因子定向诱导为ECs,并对细胞进行鉴定。结果经鉴定,HBMCs在特定环境下分化为了ECs。结论以HBMCs诱导的ECs作为种子细胞,具有可行性。Objective To investigate whether the method on incubating human bone mesenchymal stem cells（HBMCs） into ECs is feasible. Methods HBMCs were separated from human bone marrow and cultured. Then they were incubated into ECs in a special condition. The cultured ceils were identified by morphological traits,the immunohistochemical method and surface markers. Results It was proved that the HBMCs was incubated into ECs in a special condition. Conclusion The ECs derived from HBMCs can be the ideal seeding ceils to fabricate tissue- engineering homologous valved conduits.

关 键 词：人骨髓间充质干细胞 内皮细胞 种子细胞 实验 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]