金思维对老年性痴呆大鼠海马CA1区GAP-43表达的影响  

Influence of Jinsiwei (GETO) on GAP-43 expression of hippocampal CA1 in rats with Alzheimer’s disease

作　　者：徐意[1] 田金洲[1] 时晶[1] 姬志鹃[2] 尹军祥[1] 赵志炜[2] 李小黎[2] 盛树力[2] 

机构地区：[1]北京中医药大学东直门医院老年医学研究所,北京100700 [2]首都医科大学宣武医院,北京100053

出　　处：《北京中医药大学学报》2006年第3期165-167,F0003,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：教育部科学技术研究重大项目(No.10408);国家重点基础研究发展计划(973计划)项目(No.2003CB517104)

摘　　要：目的观察β淀粉样肽(Aβ)对大鼠海马CA1区GAP-43表达的改变及中药金思维对其的影响。方法应用凝聚态Aβ1-42在大鼠海马内注射以建立老年性痴呆(AD)动物模型;4周后取脑冰冻切片,采用免疫组化法和计算机图像分析技术测定海马CA1区GAP-43阳性神经元数目及光密度值。结果海马CA1区GAP-43阳性细胞数模型组和正常组(分别为52.33±28.45和59·60±22.84)相比无显著差异;金思维组(77.06±14.66)与模型组和多奈哌齐组(51.26±22·44)相比均有显著性差异(P<0.01)。海马CA1区GAP-43阳性细胞OD值模型组和正常组(分别为92.77±7.37和71·30±11.65)相比有显著差异(P<0·01);金思维组(75.44±5.29)与模型组比较有显著差异(P<0.01);而与多奈哌齐组(73.09±5·18)相比无显著性差异(P>0.05)。结论金思维可使老年性痴呆模型大鼠海马区GAP-43的表达量增加,为金思维促神经生长、改善突触可塑性和学习记忆功能的分子机制提供了新的内容。Objective To observe the changes of GAP-43 expression in hippocampal CA1 induced by 1-42 β-amyloid （ Aβ1-42 ） and the influence of Jinsiwei （GETO）. Methods To establish the Alzheimer＇ s disease （AD） model by injection of agglomerated Aβ1-42 in rat hippocampus. After 4 weeks the frozen section was taken from rat brain and the number of positive neurons of GAP-43 and optical density （OD） value in hippocampal CA1 were detected with immunohistochemistry and computer image analysis technique. Results There was no significant difference between the model group and normal group in the number of positive neurons of GAP-43 in hippocampal CA1. There was a significant difference （ P 〈 0.01 ） between the GETO group and model group, and it was the same between the GETO group and donepezil group （P 〈0.01 ）. There was a significant difference （P 〈0.01 ） between the model group and normal group in OD value of positive neurons of GAP-43 in hippocampal CA1, and it was the same between the GETO group and model group （P 〈0.01 ）. But there was no significant difference between the GETO group and donepezil group. Conclusion Jinsiwei （GETO） could improve the expression of GAP-43 of hippocampus in AD rats, which furnished new information about the molecular mechanism of GETO＇s effects of promoting nerve growth, improving synaptic plasticity, and the ability of learning and memory.

关 键 词：金思维 老年性痴呆 轴突生长 生长相关蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]