牡荆素鼠李糖苷对内皮细胞血管舒缩因子表达影响的实验研究  

Effect of vitexia-rhamnoside(V-R) on vasomotor factors expression of endothelial cell

作　　者：朱晓新[1] 李连达[2] 刘建勋[2] 刘志云[2] 马雪英[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国中医科学院西苑医院,北京100091

出　　处：《中国中药杂志》2006年第7期566-569,共4页China Journal of Chinese Materia Medica

基　　金：科技部1035计划项目(95-z-10)

摘　　要：目的:观察牡荆素鼠李糖苷对缺氧-再给氧损伤内皮细胞产生血管舒缩因子表达的影响。方法:采用脐静脉内皮细胞培养的方法,以缺氧再给氧造成内皮细胞损伤,分别以放射免疫、Griess法、免疫组化法测定细胞培养上清中内皮素-1(ET-1)、一氧化氮(NO)、细胞内一氧化氮合酶(NOS)活性;RT-PCR方法测定细胞内ET-1,NOS mR-NA的转录。结果:不同浓度的牡荆素鼠李糖苷均可明显提高内皮细胞NOS mRNA的表达和NOS的活性;使内皮细胞血管舒张因子NO产量明显增加;缩血管因子ET-1 mRNA表达及ET-1明显减少。结论:牡荆素鼠李糖苷能通过蛋白及基因水平有效地调节缺氧再给氧损伤血管内皮细胞产生的血管活性物质的表达。Objective： To observe the influence of vitexia-rhamnoside （V-R） on vasomotor factor expression of endothelial cell （EC） damaged by hypoxia and reoxygenation. Method： The cultured human umbilical vein endothelial cells （HUV ECs） were, subject to ischemia and reperfusion following hypoxia and reoxygenation. The levels of ET-1, NO and NOS intracellular in culture supertanants were measured by radioimmunity, Griess and immunohistochemistry, respectively. And the gene expressions of ET-1 and NOS intracellular were measured by reverse transcriptase-polymerase chain reaction. Result： V-R at different doses markedly increased the gene expression and activity of NOS, enhancedthe level of vaso-dilating factor NO, and significantly decreased the gene expression and production of vaso-constricting factor ET-1 of EC. Conclusion： We have demonstrated that V-R had the regulatory effect on the expression of vaso-active substances of EC damaged by hypoxia and reoxygenation in the levels of protein and gene transcription of cytokines.

关 键 词：牡荆素鼠李糖苷 内皮细胞 缺氧再给氧损伤 血管舒缩因子 

分 类 号：R285.5[医药卫生—中药学]