星点设计-效应面法优化蟾酥固体脂质纳米粒制备工艺  

Optimization preparation of chansu-loaded solid lipid nanoparticles by central composite design and response surface method

作　　者：杨勇[1] 奉建芳[1] 张慧[2] 罗杰英[3] 

机构地区：[1]上海医药工业研究院中药研究所 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]湖南中医学院药学院,湖南长沙410007

出　　处：《中国中药杂志》2006年第8期650-653,共4页China Journal of Chinese Materia Medica

基　　金：国家"863"计划项目(2001AA218011);上海市纳米科技与产业发展促进中心项目(0352nm122)

摘　　要：目的:优化蟾酥固体脂质纳米粒的制备工艺。方法:以冷却-匀质法制备固体脂质纳米粒,以平均粒径、包封率、载药量及总评“归一值”为评价指标,采用星点设计考察辅料山榆酸甘油酯用量、注射用大豆磷脂用量、泊洛沙姆188用量3因素对制备工艺的影响,对结果进行多元线性和二项式拟合,效应面法选取最佳工艺条件进行预测分析。结果:从复相关系数上看,各指标二项式拟合方程均优于多元线性回归方程,根据优化工艺制得蟾酥固体脂质纳米粒的平均粒径为71.5 nm,包封率为92.45%,载药量为5.26%。结论:优选的蟾酥固体脂质纳米粒制备工艺稳定可行,包封率高,可用于生产。Objective： To optimize the formulation of chansu-loaded solid lipid nanoparticles（Cs-SLN）. Meflaod： Cs-SLN was prepared by cold homogenization technique. The effects of influence factors such as the weight of compritol 888 ATO, soybean lecithin and poloxamer 188, on mean diameter, entrapment efficiency, drug loading and overall desirability were investigated by using central composite design and response surface method. The data were imitated using multi-linear equation and second-order polynomial equation. Result： The latter was prior to the former considering from multiple correlation coefficients. Under the optimal conditions, the mean diameter, entrapment efficiency, drug loading of the Cs-SLN were 71.5 nm, 92.45% and 5.26%. Conclusion： The optimized preparation technique for Cs-SLN is stable, feasible and high inclusion rate. It can be used for production of Cs-SLN.

关 键 词：蟾酥 固体脂质纳米粒 星点设计 效应面法 

分 类 号：TQ461[化学工程—制药化工]