针刺手厥阴心包经穴对缺血/再灌注大鼠心肌离子泵的影响  

Effect of acupuncture at acupoints of hand-Jueyin (手厥阴心包经穴) on activities of Na^+-K^+-ATPase and Ca^(2+)-Mg^(2+)-ATPase in rats with myocardial ischemia/reperfusion injury

作　　者：田岳凤[1] 李雷勇[2] 王军[1] 靳聪妮[1] 王荣[1] 郝重耀[1] 

机构地区：[1]山西中医学院针推系,山西太原030024 [2]山西医科大学第二医院,山西太原030024

出　　处：《中国中西医结合急救杂志》2006年第2期67-69,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目(30371803);山西省自然科学基金资助项目(20041123)

摘　　要：目的:观察针刺心包经穴对大鼠心肌缺血/再灌注损伤过程中心肌细胞Na+K+ATP酶和Ca2+Mg2+ATP酶活性的影响,探讨其心肌保护作用的机制。方法:50只大鼠随机分为5组。假手术组只穿线不结扎冠状动脉,其余各组制备缺血/再灌注动物模型,其中3个针刺穴位组分别于电针大鼠内关穴、郄门穴或支沟穴20min后,结扎冠状动脉左前降支40min,心电图监测,再电针相应穴位20min,恢复灌流。假手术组于穿线后100min、模型组和各针刺穴位组于再灌注60min摘取心脏,取心室肌制备心肌细胞膜,定磷法观察Na+K+ATP酶和Ca2+Mg2+ATP酶活性的变化。结果:针刺心包经穴(内关、郄门)组Na+K+ATP酶和Ca2+Mg2+ATP酶活性均明显升高,与模型组比较差异均非常显著(P均<0.01)。结论:针刺手厥阴心包经穴可提高Na+K+ATP酶和Ca2+Mg2+ATP酶活性,减轻心肌细胞钙超载程度,有效地保护心肌。Objective： To study the effects of acupuncture at the acupoints of hand-Jueyin （手厥阴心包经穴） on the activities of Na^＋- K^＋- ATPase and Ca^2＋- Mg^2＋ ATPase during the process of ischemia/ reperfusion （I/R） iniury in rats and investigate its mechanism. Methods： Fifty rats were randomly divided into five groups with 10 rats in each group. In the sham-operated group, a thread was passed through the artery, but I/R model was not established. The other groups were replicated I/R model, in which the three acupuncture groups were given acupuncture at the acupoints of Neiguan （内关穴）, Ximen （郄门穴 ） or Zhigou （支沟穴） respectively for 20 minutes, followed by ligation of the left anterior descending branch of the coronary artery for 40 minutes with electrocardiogram monitoring, afterwards, the acupoints were acupunetured again for 20 minutes, and then reperfusion was performed for 60 minutes. The myocardial tissue samples （below the ligation site） of the left cardiac ventricle were processed into cell membranes, and the two kinds of ATPase were examined by the method of fixing phosphorus. Results ： In the acupuncture groups, the activities of Na^＋- K^＋- ATPase and Ca^2＋- Mg^2＋- ATPase were increased obviously compared with that of the model group （all P〈0.01）. Conclusion： Acupuncture at the aeupoints of hand-Jueyin can obviously improve the activities of Na^＋ K^＋ ATPase and Ca^2＋ Mg^2＋-ATPase. Thus, it may reduce the degree of intracellular calcium overload, and protect myocardial performance.

关 键 词：缺血/再灌注损伤 心肌 NA^+-K^+-ATP酶 CA^2+-MG^2+-ATP酶 针刺 手厥阴经穴 

分 类 号：R245[医药卫生—针灸推拿学]