易喘平胶囊对哮喘模型小鼠气道黏液高分泌的抑制作用  

Inhibitory effect of Yichuanping capsule (易喘平胶囊) on the airway mucus overproduction in asthmatic mice

作　　者：宋立强[1] 马战平[1] 吴昌归[1] 李妍[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710033

出　　处：《中国中西医结合急救杂志》2006年第2期86-88,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：陕西省中医药科研基金资助项目(0386)

摘　　要：目的:观察易喘平胶囊(补肾活血法)对哮喘气道黏液高分泌的抑制作用及机制。方法:将32只雄性BALB/c小鼠随机分为正常对照组、哮喘组、地塞米松组和易喘平胶囊组(n=8),制备卵蛋白致敏的哮喘模型,高碘酸希夫(PAS)法检测各组小鼠气道黏膜杯状细胞数量及黏液分泌情况,逆转录聚合酶链反应(RT PCR)检测肺组织黏液蛋白MUC5AC mRNA的水平,检测支气管肺泡灌洗液(BALF)中嗜酸粒细胞数及细胞总数,酶联免疫吸附法(ELISA)检测肺组织匀浆液中白细胞介素4(IL4)和γ干扰素(IFNγ)的含量。结果:与哮喘组相比,易喘平胶囊组和地塞米松组小鼠小支气管上皮杯状细胞数量明显减少,管腔内黏液量也减少;同时后两组的MUC5AC mRNA水平也显著降低(P均<0.01)。易喘平胶囊组和地塞米松组BALF中嗜酸粒细胞数和细胞总数,以及肺组织中IL4含量均低于哮喘组(P均<0.01),而IFNγ含量有所提高(P均<0.01)。结论:易喘平胶囊对哮喘气道黏液高分泌具有一定抑制作用,调节辅助T淋巴细胞Th1/Th2的平衡、降低气道炎症程度是其发挥作用的机制之一。Objective： To evaluate the inhibitory effect of Yichuanping capsule （易喘平胶囊） on the airway mucus overproduction in asthmatic mice and its mechanism. Methods： Thirty-two male BALB/c mice were randomly divided into normal control group, asthma group, dexarnethasone group and Yichuanping capsule （YC） group （n= 8 in each group）. Asthmatic mouse model was established by intraperitoneal injection of ovalbumin. The number of goblet ceils and secretion of mucus were detected with periodic acid-Schiff （PAS） staining, and mucus protein MUC5AC mRNA was detected with reverse transcription-polymerase chain reaction （RT- PCR）. Number of total cells and eosinophil in bronchoalveolar lavage fluid （BALF） were counted with special staining. The contents of interleukin - 4 （IL - 4） and γ- interferon （IFN -γ） in lung tissue were assessed by enzyme-linked immunoadsorbent assay （ELISA）. Results： Compared with asthma group, the number of goblet cells, MUC5AC mRNA levels and secretion of mucus in airway were reduced in dexamethasone group and YC group（all P〈0. 01）. The number of total cells and eosinophil in BALF, and the contents of IL - 4 in lung in dexamethasone group and YC group were lower than those in asthma group （all P〈0.01）, but IFN-γ contents increased （P〈 0.01）. Conclusion： Yichuanping capsule shows the inhibitory effect on the airway mucus overproduction in asthma, and one of the mechanisms is regulating the ratio of Th1/Th2 cell and inhibiting the airway inflammation.

关 键 词：易喘平胶囊 哮喘 气道 黏液分泌 炎症 气道 

分 类 号：R285[医药卫生—中药学]