5/6肾切除大鼠血清瘦素水平变化及其与肾小球硬化关系  

Relationship Between Changes of Serum Leptin Levels and Degree of Glomerulosclerosis in Rats After 5/6 Nephrectomy

作　　者：杨利才[1] 张道友[1] 朱新俭[1] 汪裕伟[1] 高潮清[1] 徐海红[1] 

机构地区：[1]皖南医学院弋矶山医院肾内科,241001

出　　处：《放射免疫学杂志》2006年第2期87-90,共4页Journal of Radioimmanology

基　　金：安徽省高等学校青年教师科研基金项目(2004jq192)

摘　　要：目的:观察5/6肾切除大鼠血清瘦素(leptin)水平及其与肾小球硬化指数(GSI)、肾小球局部转化生长因子-β1(TGF-β1)表达、细胞外基质(ECM)的关系。方法:选用SD雄性大鼠14只,其中8只通过5/6肾切除法制造慢性功能衰竭模型,另6只为假手术组作为正常对照。术后第6周末各组大鼠进行血清肌酐(Scr)、尿素氮(BUN)及leptin的测定,处死大鼠,取出肾组织进行病理组织形态学观察,并采用免疫组织化学方法检测肾小球TGF-β1、Ⅳ型胶原(ColⅣ)及纤维连接蛋白(FN)表达。并对血清leptin水平与GSI、TGF-β1、ColⅣ及FN之间的关系进行相关性分析。结果:5/6肾切除大鼠血清瘦素(leptin)水平显著高于假手术组(14.88±1.46ng/mlvs10.84±2.67ng/ml,P<0.01),并与GSI、TGF-β1、ColⅣ及FN呈显著正相关(P<0.01)。结论:高瘦素血症可能是引起肾小球硬化的机理之一。Objective To investigate the relationship between changes of serum leptin levels and glomeruloselerosis index, TGF -β1 expression at glomerulus as well as degree of ECM proliferation in rats after 5/6 nephrectomy. Methods Eight rats anderwent two - stage 5/6 nephrectomy to establish a pregressive glomerulosclerosis model. Six weeks after the second operation, the rats were sacrificed, the renal tissae was examined with routine pathological sections for study of glomerulosclerosis as well as with immuno - histo chemical studies for TGF -β1, collagen Ⅳ and fibronectin expressions. Six rats underwent sham operation served as controls. Serum creatinine, urea nitrogen and leptin levels were measured in all these animals. Results The serum levels of leptin were significantly higher in rats after 5/6 nephrectomy than the rats with sham operations （ 14.88 ± 1.46ng/ml vs 10.84 ± 2.67ng/ml, P 〈 0.01 ）. Serum levels of leptin were positively correlated with the glomerulosclerosis index and collagen Ⅳ, fibronectin expressions （ P 〈 0.01 ）. Conclusion Hyperleptinemia may be one of the causative factors of development of glomeruloselerosis.

关 键 词：瘦素 肾小球硬化 转化生长因子-Β1 

分 类 号：R692.6[医药卫生—泌尿科学] R692[医药卫生—外科学]