新药肝龙胶囊对雏鸭体内鸭乙型肝炎病毒的抑制效果  

Experimental Research on Antiviral Activity of Ganlong Capsule Against Duck Hepatitis B Virus

作　　者：杜一民[1] 李树楠[1] 陈鸿珊[2] 李壮[2] 

机构地区：[1]大理学院药学院,云南大理671000 [2]中国医学科学院医药生物技术研究所,北京100050

出　　处：《大理学院学报（综合版）》2006年第4期6-8,共3页Journal of Dali University

基　　金：国家计委95重点攻关项目(NO.96-20-09)

摘　　要：目的:研究新药肝龙胶囊对鸭乙型肝炎病毒的体内抗病毒作用。方法:以鸭乙型肝炎病毒(DHBV)静脉注射感染雏鸭为模型,于感染第7天后分组灌喂肝龙(GL)胶囊浸膏0.5、1.5、3.0g.kg-1.d-1及生理盐水(对照组),阳性对照组腹腔注射无环鸟苷(0.4g.kg-1.d-1)或磷羧基甲酸钠(0.5g.kg-1.d-1)。给药每天2次,连续10d。分别于给药前、给药后第5天、第10天和停药后第3天取血清,采用斑点杂交方法检测不同时间鸭血清DHBV-DNA,观察其动态变化,以病毒抑制率为疗效指标。结果:三次重复的结果表明,与给予生理盐水的病毒对照组比较,肝龙的三个不同剂量组均有不同程度的疗效,且不同剂量组之间有一定的量效关系,其中GL3.0g.kg-1.d-1组疗效明显(P<0.01),与阳性对照组无环鸟苷或磷羧基甲酸钠组的疗效无显著性差异,而且作用持续时间较长。结论:肝龙(GL)胶囊可抑制DHBV感染雏鸭体内DHBV-DNA的复制。Objective： To observe the antiviral activity of Ganlong capsule against duck Hepatitis B virus （DHBV） in vivo. Methods： The duck Hepatitis B model was made by injecting DHBV into the veins of one-day-old Beijing duck, In the seventh day after infected by DHBV, Ganlong capsule was given by intragastric administration for 10 days in group Ⅰ （0.5g, kg^-1·d^-1 ）, group Ⅱ （ 1.Sg.kg^-1·d^-1 ）, group Ⅲ （3.0g·kg^-1·d^-1 ）, bid, and detected the levels of DHBV-DNA in the duck blood sere obtained separately in the day before giving Ganlong capsule, the fifth day and tenth day after giving Ganlong capsule, and the third day after withdrawing Ganlong Capsule, by serum dot-blot hybridization. Tbe trial was contrasted with acyclovor（ACV） or phosphonoformate（PFA ）, Results： The resuhs of three time experiments showed that the levels of sera DHBV-DNA were decreased in the treatment groups of Ganlong, anN had the relationship of dose-effect. The group Ⅲ （3.0g·kg^-1·d^-1） inhibited significantly（P〈0.01 ） the serum DHBV-DNA, and after stopping the treatment for 6 days, scram DHBV-DNA level did not return significantly, and the inhibit elM：is were no different between with the treatment group of phosphonoformate（PFA ） of aeyclovor（ ACV ）. Conclusion： These results suggested that Ganlong capsule markedly inhibited duck hepatitis virus rcplication in vivo.

关 键 词：肝龙胶囊 鸭乙型肝炎痛毒 鸭乙肝动物模型 

分 类 号：R944.5[医药卫生—药剂学]