青翘超临界CO_2萃取及连翘苷转移的研究  

Study on Unripen Fructus Forsythiae Extracted by SPE-CO_2 and the Diversion of Phillyrin

作　　者：肖会敏[1] 王四旺[1] 王剑波[1] 谢艳华[1] 孙玲变[1] 薛丽[2] 

机构地区：[1]中国人民解放军第四军医大学药物研究所,陕西西安710033 [2]中国人民解放军第518医院,陕西西安710043

出　　处：《解放军药学学报》2006年第2期124-126,155,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的 以收油率为指标，优选超临界CO2萃取青翘挥发油工艺条件；检测提取物残渣中连翘苷含量，比较和分析活性成分连翘苷的转移率。方法 正交试验设计5因素3水平萃取青翘挥发油；HPLC测定连翘苷含量，色谱柱Phenomenex Luna 5μm C18（2）100A 250mm×4．6mm，流动相为乙腈-水（25：75），检测波长为277nm。结果 青翘挥发油最佳萃取条件是萃取压力30mPa，萃取温度32℃，分离压力6．6mPa，分离温度30℃，粒度为2．0mm；青翘挥发油中未检出连翘苷；提取物残渣中连翘苷含量为1．30～6．40mg·g^-1。结论 青翘挥发油提取率为0．94％～7．85％；因连翘苷富集于提取物残渣中，而挥发油中其含量甚微；残渣物因其富集连翘苷所以具有再次开发与研究价值。Aim To optimize SPE-CO2 conditions of essential oil from tmriped Fructus Forsythiae by orthogonal design,determine the content of Phillyrin in the remainders of extractions, and compare and analyze the diversion ratio of active components of Phillyrin. Methods Orthognnal design of 5 factors at 3 levels was applied for extraction from Qing Fructus Forsythiae. HPLC was used to detemaine the content of Phillyrin in the remainders of extractions, lama 5μm C18（2） 100A 250mm × 4.6mm was used, acetonitrile-water（25：75） as mobile phase and the wavelength of UV detector was 277mn.Results The optimal condition was extraction pressure 30mPa, extraction temperature 32℃, separature pressure 6.6mPa,separature temperature 30℃, 2.0mm. There wasn＇t Phillyrin in the essential oil. The content of Phillyrin was 1.30 ～ 6.40mg·g^-1 in the remainders of extractions. Conclusion The extraction percentage of unriped Fructus Forsythiae was 0.94% ～ 7.85% ;Phillyrin was concentrated in the remainders of extractions and was microamount in the essential oil,the remainders of extraction for enriching Phillyrin need to be further developed and studied.

关 键 词：连翘 超临界萃取 挥发油 正交试验 含量测定 

分 类 号：R917[医药卫生—药物分析学]