RP-HPLC法测定止咳橘红口服液中柚皮苷的含量  

Determination of Naringinin in Zhike Juhong oral Liquid by RP-HPLC

作　　者：黄少彬[1] 李笑芬[1] 张超 吴锦芳[1] 

机构地区：[1]广州市花城制药厂,广东广州510555 [2]广州市普度医药科技有限公司,广东广州510636

出　　处：《解放军药学学报》2006年第2期138-140,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的 建立RP-HPLC法测定止咳橘红口服液中有效成分柚皮苷含量的方法。方法 采用HPLC法，色谱柱为C18柱，流动相为甲醇-冰乙酸-水（70：3：130），检测波长为283nm。结果 柚皮苷线性范围13．0～130μg。ml^-1，r=0．999 9（n=5）；止咳橘红口服液中柚皮苷回收率为99．0％（n=9），RSD为1．06％。结论 测定方法简便、稳定，能有效地控制该制剂的质量。Aim To establish an RP-HPLC method for detemaination of naringin in Zhike Junhong oral Liquid. Methods The HPLC method was adopted to C18 column, with methanol-acetic acid-water（70： 3：130） as the mobile phase, detection wavelength was set at 283nm. Results In the calibration curve for naringin was linear over the range of 13.0~130μg·ml^-1. The average recovery was 99.0% with RSD 1.06%. Conclusion The method is simple, convenient and reliable for oualitv control of the liquid.

关 键 词：止咳橘红口服液 柚皮苷 RP-HPLC 

分 类 号：R917[医药卫生—药物分析学]