不同钙离子浓度对日本沼虾感光器细胞超微结构的影响  

Effects of Different Calcium Concentration on the Retinular Cell in the Shrimp Macrobrachium nipponense

作　　者：罗永婷[1] 盛春[1] 张赪蕾[1] 钱忠英[1] 张慧绮[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《细胞生物学杂志》2006年第2期233-238,共6页Chinese Journal of Cell Biology

基　　金：上海市教委基金资助项目(No.03DZ05)~~

摘　　要：为了进一步研究细胞外钙离子浓度变化对甲壳动物感光细胞超微结构的影响,应用透射电子显微镜显示了日本沼虾感光细胞,在暗适应时高钙离子浓度中温育的感光器细胞的感杆束直径下降,微绒毛排列零乱;多囊体、板膜体数量增加;色素颗粒散布在细胞质中,呈现出光适应的结构特征。而温育在低钙离子溶液和生理溶液中的感光器细胞结构相同,呈现出暗适应的结构特征。另外,细胞器中储存的钙离子也受细胞外钙离子浓度的影响,在高钙离子溶液中温育后细胞器储存的钙离子量增加,膜下储泡囊、多囊体、线粒体、色素颗粒等细胞器中的焦锑酸钙结晶颗粒比温育在低钙溶液中的细胞明显增多。结果显示,细胞外钙离子浓度变化引起细胞内钙离子浓度变化,从而影响感光器细胞的结构而影响其生理功能。In order to further study the effects of Crustacea＇s retinular cells under different calcium concentration, the dark adapted retinular cells of the photoreceptors in the shrimp （Macrobrachium nipponense）, that were incubated in physiological saline and in presence of different calcium concentrations, were studied with electron microscopy. The results showed that in presence of 50 mmol/L calcium, the fine structure of the retinular cells had the characters of the cells in light adaptation. The diameter of the rhabdoms reduced extremely. The microvillus that composed the rhabdoms arranged in disorder. Cells contained more multivesicular bodies and more lamellar bodies. The pigment granules were more and located in the whole cells. The retinular cells that were incubated in physiological saline （10 mmol/L） or in low calcium saline （50 mmol/L EGTA and 2 mmol/L） had same fine structures that showed the characters in dark adaptation. The calcium concentrations out of cells effected not only on the fine structures of the retinular cells but also on the calcium deposited in the organelles. In higher calcium solution that deposited more calcium in the organelles. The number of the deposits of calcium antimonate that were found in the organelles such as perirhabdomal vacuole, mitchondria, multivesicular body and pigment granule were more in higher calcium solution than in low calcium solution. Our studies concluded that the calcium out of retinular cells influenced the calcium in retinular cells, and those influence the function of the retinular cells by effects on the fine structure of the cells.

关 键 词：日本沼虾 感光器 钙离子 超微结构 

分 类 号：Q952[生物学—动物学] Q436