人牛精浆相关蛋白的亚细胞定位及生物活性  

Biological Activity and Subcellular Localization of HBRP

作　　者：张杰[1] 刘莹[1] 宗志红[1] 宿文辉[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001

出　　处：《细胞生物学杂志》2006年第2期243-247,共5页Chinese Journal of Cell Biology

基　　金：国家自然科学基金资助项目(No.30170987)~~

摘　　要：利用红色荧光蛋白表达载体pDsRed1-N1与人睾丸中牛精浆相关蛋白基因(hbrp)重组为pDsRed1-N1/hbrp,真核表达载体pcDNA3.1-myc-his与hbrp重组为pcDNA3.1-myc-his/hbrp,分别转染HEK293细胞,建立稳定的真核表达细胞系。在荧光显微镜下观察HBRP的亚细胞定位,并用放射自显影检测该细胞系的蛋白激酶C(PKC)活性。HBRP定位在细胞膜及胞浆近膜处;HBRP对PKC活性有明显的抑制作用。从而确定了人新的精子结合蛋白HBRP在细胞内的定位,并认定了HBRP为有生物活性的功能蛋白。Two recombinant eukaryotic expression vectors, pDsRedl-N1/hbrp and pcDNA3.1-myc-his/ hbrp, have been constructed and used to transfect HEK293 cell to form a stable expression cell line. The cellular localization and biological function of HBRP were investigated using fluorescence microscope and radioautography method, respectively. The experimental results showed that HBRP was a functional protein which localizes in the cellular membrane and cellular plasma near the membrane, and had a notable inhibitory effect on the PKC activity.

关 键 词：人牛精浆相关蛋白 牛精浆蛋白 亚细胞定位 蛋白激酶C活性 

分 类 号：R341[医药卫生—基础医学]