2株SARS相关冠状病毒主要结构蛋白基因的多态性分析  

Sequence polymorphism in the main structural protein genes of two strains of Severe Acute Respiratory Syndrome(SARS)-associated coronaviruses

作　　者：齐艳[1] 包其郁[1] 田薇 徐建成 

机构地区：[1]温州医学院生物医学信息研究所,浙江温州325035 [2]杭州华大基因研发中心,浙江杭州310008

出　　处：《温州医学院学报》2006年第2期125-127,130,共4页Journal of Wenzhou Medical College

基　　金：温州市科技局科研基金资助项目(Y2003A005)

摘　　要：目的:分析2株SARS相关冠状病毒(Severeacuterespiratorysyndrome-associatedcoronavirus,SARS-CoV)HK21378和BJ104的主要结构蛋白基因序列的多态性以及蛋白质结构变化与功能的关系。方法:提取组织培养物中的SARS-CoV基因组RNA,对RT-PCR产物进行测序;以GZ02为参照序列,对蛋白质编码序列进行基因多态性分析和蛋白质结构与功能关系分析。结果:①与SARS-CoVGZ02株相比较,HK21378和BJ104主要结构蛋白编码序列共有14个变异位点,其中9个变异位点为两者共有3,个变异位点见于HK21378,2个变异位点见于BJ104;与GenBank数据库已发表的103株SARS-CoV基因组数据比较,变异位点22901(T→G)为BJ104特有,未见于已发表的其他SARS-CoV基因组中;在14个多态位点中,有5个多态位点为同义突变,其余9个为非同义突变。②14个变异位点中有11个变异发生在S蛋白编码区,其碱基变异频率为3.7/kb;2个见于M蛋白编码区,其碱基变异频率为3.0/kb;1个见于N蛋白编码区,碱基变异频率为0.8/kb;而E蛋白编码区未见变异位点。结论:SARS-CoV的主要结构蛋白编码区容易发生变异,尤其是膜表面S蛋白和M蛋白编码区。Objective： To analyze the polymorphism sequences of 2 SARS-associated coronaviruses,HK21378 sequence variation and the protein function.Methods： of the main structural protein-encoding and BJ104,and the relation ship between RT-PCR was performed to amplify the main structural protein genes.Phred/Phrap/Consed was used to assemble the sequences. Alignment was performed with Clustal W1.8.Results： ① Compared with the sequences encoding the spike protein, the envelop protein, the membrane protein and the nucleocapsid protein of SARS-CoV GZ02, there were 14 variant loci in those sequences of HK21378 and BJ104. Both HI（21378 and BJ104 had 9 loci in common and the other 5 were different from each other, of which HK21378 and BJ104 had 3 and 2, respectively.Compared with 103 SARS-CoV genome sequences available in GenEank, one mutation （22901,T--G） unique to BJ104 was identified. Among all the fourteen variant loci, nine substitutions were predicted to lead amino acid changes （non-synonymous mutation） and five were loci of synonymous mutations.② Among 14 variant loci, 11 were identified in the region encoding S protein with the mutation rate of 3.7/kb, 2 loci were identified in the region encoding M protein with the mutation rate of 3.0/kb and 1 variation in N protein encoding region with the mutation rate of 0.8/kb. No polymorphic locus was identified in the region encoding E protein. Conclusion： The sequences encoding structural protein is easy to mutate, especially in the regions encoding S and M protein.

关 键 词：SARS相关冠状病毒 结构蛋白基因 多态性 

分 类 号：R373.1[医药卫生—病原生物学]