泽苔草交配系统的RAPD分析  

RAPD Analysis for Mating System in Caldesia parnassifolia

作　　者：董贞荣[1] 陈进明[1] 王青锋[1] 

机构地区：[1]武汉大学生命科学学院植物系统学与进化生物学研究室,武汉430072

出　　处：《武汉植物学研究》2006年第2期167-170,共4页Journal of Wuhan Botanical Research

基　　金：国家重点基础研究发展规划(973)项目(G2000046805)资助;国家自然科学基金资助项目(30370098)

摘　　要：利用RAPD显性标记位点对泽苔草(Caldesia parnassifolia)湖南茶陵湖里居群进行了交配系统研究。采用10个家系共240个子代样品测定了该居群花粉库基因频率、固定指数(F)、多位点异交率(tm)和单位点异交率(ts)。结果显示该居群异交率处于较高的水平(tm=1.008),多位点异交率与单位点异交率差异不显著(tm-ts=-0.021),固定指数(F)的估计值小于0(F=-0.200),表明该居群双亲近交不明显,而以异交为主。这可能是受居群密度、传粉者的活动能力等因素影响的结果。Outcrossing rate in an open-pollinated breeding population （Huh population, Chaling, Hunan Province） of Caldesia parnassifolia was estimated by the dominant RAPD makers using 10 open-pollinated progeny arrays of 24 individuals. The muhilocus outcrossing rate （tm） was 1. 008 and the single-locus outcrossing rate （ ts ） was 1. 028. The multilocus outcrossing rate did not significantly differ from the single-locus outcrossing rate （ tm - ts = - 0.021 ）. The fixation index （F） was - 0.200. The estimates of tm , the ts and F utilizing windows version of MLTR program clearly indicate that outcrossing is predominant in C. parnassifolia open-pollinated breeding population. This is possibly influenced by factors such as plant density, the adaptabihty of pollinator and so on.

关 键 词：泽苔草 交配系统 异交率 克隆植物 RAPD 

分 类 号：Q948[生物学—植物学]