豚鼠鼓室明胶海绵吸附给药外淋巴液中甲泼尼龙的含量测定  

Determination of methylprednisolone in the scala tympani perilymph of guinea pig after administration by gelatin sponge with methylprednisolone solution

作　　者：张顺国[1] 陈敏玲[1] 李方[1] 杨军[1] 侯东明[1] 吴皓[1] 

机构地区：[1]上海第二医科大学附属新华医院,上海200092

出　　处：《中国现代应用药学》2006年第2期139-141,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：上海市科委科技攻关计划项目(034119819)

摘　　要：目的建立豚鼠外淋巴液和血浆中甲泼尼龙的含量测定方法。方法采用浸满甲泼尼龙溶液的明胶海绵颗粒置于圆窗龛内，采集给药1～6h后鼓阶外淋巴液。HPLC色谱条件为，ZORBAX Eclipse XDB—C18色谱柱（4．6mm×250mm，5μm），流动相为7．56mmol／L硫酸铵-乙腈（68：32），流速1．0mL·min^-1，柱温20℃，紫外检测波长243nm。结果甲泼尼龙的线性范围为0．10～5．0mg·L^-1（r=0．9998）。最低检测限为5．72ng，日内和日间RSD分别为1．91％和2．57％。外淋巴液中甲泼尼龙的消除半衰期为1．40h。结论方法灵敏、准确，重现性好，可用于外淋巴液中甲泼尼龙的测定。OBJECTIVE To establish a method for determining the concentration of methylprednisolone in perilymph and serocym of guinea pig. METHODS Placing a granule gelfoam soaked with methylprednisolone in the niche of round window of guinea pig, perilymph of the scala tympani and serocym were sampled 1 - 6h after administrations. HPLC conditions： ZORBAX Eclipse XDB-C18 column as stationary phase, 7.56mmol · L^-1 ammonium sulfate-acetonitrile （68： 32） as mobile phase with the flow rate of 1.0mL · min^-1. The temperature of column was at 20℃, the wavelength of detection was 243nm. RESULTS The calibration curve was linear within the concentration range of 0.10 -5.0mg · L^-1 ,r = 0.999 8, the limit of detection was 5.72ng, the RSD values of within-day and between days were 1.91% and 2.57%. The elimination half life of methylprednisolone in perilymph of the scala tympani was 1. 40h. CONCLUSION The method appeared to be simple, accurate, reproducible and can be used to determine the concentration of methylprednisolone in perilymph and serocym.

关 键 词：甲泼尼龙 外淋巴液 高效液相色谱法 

分 类 号：R917.794[医药卫生—药物分析学]