大鼠胸主动脉球囊损伤后内膜增生的实验研究  

Experimental Study of Neointimal Formation of Balloon Injured Rat Aorta

作　　者：姚园[1] 江洪[1] 李庚山[1] 徐超[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《医学临床研究》2006年第4期520-522,共3页Journal of Clinical Research

摘　　要：【目的】制作大鼠胸主动脉球囊损伤的动物模型,观察内膜增生和内皮修复的动态变化。【方法】将32只雄性SD大鼠随机分为对照组(8只)和实验组(24只),于球囊损伤术后d7、d14和d28处死实验组动物。分别进行伊文思蓝染色观察内皮覆盖情况,增殖细胞核抗原(PCNA)免疫组化染色和组织形态学定量分析。【结果】和对照组相比,球囊损伤后内膜面积与中膜面积的比值(IA/MA)和再生内皮覆盖率呈持续性增加(P<0.01),而反映血管平滑肌增殖活性的PCNA阳性表达指数呈持续性下降(P<0.01)。【结论】内膜增生是管腔狭窄的主要因素,而内皮损伤是这一过程的始动因素。[Objective]To observe the dynamic change of neointimal formation and endothelial regeneration of balloon injured rat aorta. [Methods]Thirty-two male SD rats were randomly divided into control group and 3 experiment groups, 8 rats in each experiment group were killed on 7. 14, 28 days after injury. The endothelial regeneration rate. IA/MA ratio. PCNA expression index and morphological changes were detected. [Results] Compared with control group, the endothelial regeneration rate and IA/MA ratio increased continuously, and the PCNA expression index decreased gradually. [Conclusion]The neointimal formation is the main factor of restenosis, and the endothelial injury is the initial factor.

关 键 词：主动脉 胸/损伤 大鼠 

分 类 号：R543.16[医药卫生—心血管疾病]