群体感应机制在生防细菌筛选中的应用  

Isolation and Bio-control Effect of Bacterial Strain by Quorum Sensing Mechanism

作　　者：黄华平[1] 代鹏[1] 唐复润[1] 邓国平[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所热带作物生物技术国家重点实验室,海南海口571101

出　　处：《安徽农业科学》2006年第7期1394-1396,共3页Journal of Anhui Agricultural Sciences

基　　金：科技基础性工作和社会公益研究专项(2004DIB3J073)

摘　　要：根据群体感应机制,利用PCR技术,摸索出一套能够高效筛选生防细菌的方法。利用这一方法,从土壤中分离的1 399株细菌中筛出42株,占总分离株的3%,PDA培养基平板拮抗试验发现,其中有39株对香蕉尖孢镰刀菌有抑菌效果,占总筛选株数的95.2%。抑菌效果最好的分离株DFL059在田间试验中对香蕉枯萎病有较强的生防效果。Based on the Quorum sensing mechanism, an isolation model of bio-control of bacteria was designed with the method of PCR technique. 42 strains were isolated from 1 399 strain bacteria. The percentage was 3% in total strain. On PDA medium, 39 strains were found to suppress Fusarium oxysporum of banana in the 42 strains. The percentage was 95.2%. The DFL0159 strain was found the best strain with the antagonistic ability to Fusariurn oxyporum of banana.

关 键 词：群体感应 生防细菌 尖孢镰刀菌 

分 类 号：Q936[生物学—微生物学]