供体细胞对猪体细胞克隆胚胎早期发育的影响  

Effects of Donor Cells on in vitro Development of Porcine Cloned Embryos

作　　者：潘登科[1] 张运海[2] 孙秀柱[2] 李燕[2] 吴常信[1] 李宁[2] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《畜牧兽医学报》2006年第4期331-336,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："973"国家重点基础研究发展规划(G20000161);"863"国家高技术研究发展计划(2003AA205102);北京市自然科学基金重大项目(5030001)的资助

摘　　要：以中国农业大学实验用小型猪香猪胎儿成纤维细胞、成年耳成纤维细胞和颗粒细胞3种细胞系为供体细胞进行核移植。比较了血清饥饿法和接触抑制法处理胎儿成纤维细胞诱导进入G0/G1期的效率,发现二者差异不显著(P>0.05),血清饥饿2d和4d差异不明显,同样接触抑制2d和4d差异也不显著(P>0.05)。系统研究了影响克隆胚胎发育的供体因素:血清饥饿与否、细胞形态、细胞类型及个体差异等,结果表明:血清饥饿处理对克隆胚的早期发育没有明显的促进作用;圆形光滑细胞有利于细胞融合,对早期发育无显著影响(P>0.05);不同个体、不同类型的供体细胞对克隆胚囊胚发育率有一定的影响。Porcine fetal fibroblast cells, ear fibroblast cells and granulosa cells were used as donor in pig cloning. We compared the effects of cell cycle synchronization treatment of fetal fibroblast cells into G0/G1 by serum starvation or contact inhibition. There were no significant difference in percentage of G0/G1 between two treatments, no significant difference in percentage of G0/G1 by serum starvation for 2d and 4d, as the same when by contact inhibition. Serum starved fetal fibroblast cells or cycling growing subconfluenced cells, round and smooth vs. rough surface cells, different types of somatic ceils and fibroblast ceils of different individuals were used as nuclear donor in porcine somatic cloning. The results indicated that serum starvation could not improve the development of cloned embryos. Round and smooth cells led to a high fusion rate. Cell types of donor and different individuals impacted on the development of cloned embryos.

关 键 词：胎儿成纤维细胞 血清饥饿 细胞类型 克隆胚胎 

分 类 号：S814.8[农业科学—畜牧学]