重金属污水对木榄幼苗几种保护酶及膜脂质过氧化作用的影响  

Effects of wastewater borne heavy metals on some protective enzymes and lipid peroxidation of Bruguiera gymnorrhiza seedling

作　　者：张凤琴[1,2] 王友绍[1,2] 董俊德[1,2] 孙翠慈[1,2] 殷建平[1,2] 

机构地区：[1]中国科学院南海海洋研究所中国科学院热带海洋环境动力学重点实验室,广东广州510301 [2]中国科学院大亚湾海洋生物综合实验站,广东深圳518121

出　　处：《热带海洋学报》2006年第2期66-70,共5页Journal of Tropical Oceanography

基　　金：中国科学院知识创新工程交叉型方向性项目(KSCX2-SW-132)

摘　　要：用5个级别的人工复合重金属污水处理红树植物木榄Bruguiera gymnorrhiza幼苗,2个月后对其叶片和根系中的保护酶SOD、POD和CAT活性变化以及对膜脂质过氧化作用的影响进行分析。结果显示,当污染级别不大于5倍污水时,根系中SOD活性随污染程度的增加而增加,污染程度再升级其活性迅速下降但仍高于对照组水平;叶片中SOD活性在1倍污水时与对照组相比有所下降,随后的变化与在根系中的变化相似。POD活性在根系和叶片中的变化相对一致,均随污染级别的增加而升高;但在叶片中的变化幅度大于根系中的变化幅度。CAT活性在叶片中不受污染程度的影响,保持平稳,但在高程度污染下活性有下降趋势;而根系中CAT活性在10倍污水时达到最大值,污染程度再升级其活性下降。叶片中膜脂质过氧化作用随污染程度的增加而加剧,MDA的积累增加;但根系中MDA含量在5倍污水时最少,只为对照组的21.98%,其后随着污染程度的增加其MDA含量大幅度积累,膜脂质过氧化作用加剧。从整个试验结果分析认为,木榄在遭受重金属胁迫时具有优先保护根系以增强植物的抗重金属能力。The variance of superoxide dismutase, peroxidase and catalase activities, and the impact on lipid peroxidation in leaf and root of Bruguiera gymnorrhiza seedlings were analysed after the treatment by five levels of wastewater-borne heavy metals （Zn, Pb, Cd, Hg） for two months. The results showed that when the pollutant level was five times lower than the wastewater, the superoxide dismutase activity in root would increase with the increase of heavy metal contents; when the pollutant level was higher, the activity would rapidly decline but would not be lower than that of the contrast. The situation was similar in leaf, only that the activity declined at one time level of the wastewater compared with the contrast. The activities of peroxidase both in leaf and in root increased with the increase of pollutant level, but its range was wider in leaf than in root. The activity of catalase in root increased and reached to its maximum at 10 times level of the wastewater, then declined; while in leaf, the activity remained steady. The lipid peroxidation in leaf strengthened with the increase of wastewater-borne heavy metal contents; while it weakened in root before the pollutant level was 5 times lower than the wastewater, then strengthened with the increase of heavy metal contents. The content of MDA reached to its lowest, 21.98%, at 5 times level of the wastewater. It was concluded that Bruguiera gymnorrhiza protects its root firstly so to strengthen the resistance to heavy metals.

关 键 词：红树植物木榄 BRUGUIERA gymnorrhiza 人工复合重金属污水 SOD POD CAT 膜脂质过氧化作用 

分 类 号：Q948.8[生物学—植物学] X503.231[环境科学与工程—环境工程]