黄芩苷抑制大鼠胰岛细胞瘤细胞株增殖的分子机制研究  

Study on Molecular Mechanism of Inhibitory Effect of Baicalin on Proliferation of Insulinoma Cell Line in Rats

作　　者：孙吉平[1] 贾延劼[1] 宋健辉[1] 杨于嘉[1] 

机构地区：[1]中南大学湘雅医院儿科,长沙410008

出　　处：《中国中西医结合杂志》2006年第4期337-340,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30200128)

摘　　要：目的探索黄芩苷对大鼠胰岛细胞瘤细胞的影响及作用的分子机制。方法应用光学显微镜、MTT测定、流式细胞仪、报告基因分析以及WesternBlot等方法,探讨黄芩苷对细胞增殖和细胞周期的影响及作用分子机制。结果黄芩苷处理大鼠胰岛细胞瘤细胞后,分裂相细胞显著减少,细胞增殖受到抑制,细胞的生存率下降,随黄芩苷浓度的增加和/或作用时间的延长,抑制率呈上升趋势,并出现凋亡细胞;胰岛细胞瘤细胞株凋亡过程中caspase3活性以时间依赖性方式增高;通过流式细胞仪检测细胞周期发现,随黄芩苷处理浓度增加,S期细胞逐渐减少,从38.2%下降至9.4%,G0/G1期细胞所占百分率逐渐增加,从56.4%上升至85.9%,细胞出现G1阻滞;同时,细胞周期蛋白基因启动子活性显著下调,细胞周期蛋白表达明显降低。结论黄芩苷能诱导细胞凋亡,其作用可能与caspase3活化有关;黄芩苷还能以剂量依赖性方式和时间依赖性方式抑制胰岛细胞瘤细胞增殖,黄芩苷诱导细胞周期蛋白基因转录和表达下调可能在其中起着重要作用。Objective To investigate the effect of baicalin on insulinoma cell line and the molecular mechanism involved. Methods Light microscope, MTT assay, flow cytometry, gene analysis and Western Blot were applied to investigate the effects of baicalin on the cell proliferation, the cell cycle and the involved molecular mechanism. Results After treatment with baicalin, the number of cells in mitotic stage and the survival rate of cells obviously decreased, and cell proliferation was inhibited in a drug concentration- and acting time-dependent manner, with the appearance of apoptotic insulinoma cells. During the apoptotic process, the activity of caspase- 3 was elevated by baicalin in a time-dependent manner; with the increase of the concentration of baicalin, the number of cells in S-phase obviously decreased from 38.2% to 9.4% , while the percentage of cells in G0/G1 phase increased from 56.4 % to 85.9 %, indicating cells were arrested in G1-phase. Meanwhile, the activity of cyclin gene promoter obviously declined, and the expression of cyclin reduced remarkably. Conclusion Baicalin could induce apoptosis of insulinoma cells, which might be correlated with the activity of caspase-3, and inhibiting proliferation of insulinoma cells in a concentration- and time-dependent manner, in which the action of baicalin in down-regulating the gene transcription and expression of cyclin may play an important role.

关 键 词：黄芩苷 胰岛细胞瘤 增殖 细胞周期蛋白 

分 类 号：R285[医药卫生—中药学]