益气降浊汤对CVB3m重复感染致病毒性心肌炎小鼠TGF-β_1 mRNA表达的影响  

Effects of Yiqi Jiangzhuo Decoction on Transforming Growth Factor-β_1 mRNA Expression in Treating Ventricular Remodeling in Mice with Repetitive CVB3m Infection Caused Viral Myocarditis

作　　者：王莹威[1] 郭玲[2] 李奇虹[1] 李延[1] 

机构地区：[1]黑龙江中医药大学临床医学院,哈尔滨150040 [2]哈尔滨医科大学附属第四医院

出　　处：《中国中西医结合杂志》2006年第4期341-343,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.39670656)

摘　　要：目的探讨益气降浊汤对病毒性心肌炎转化生长因子β1(TGFβ1)mRNA表达的影响。方法建立慢性病毒性心肌炎模型,通过4次梯度倍增CVB3m病毒感染小鼠,160只雄性昆明鼠分成空白组、模型组、中药治疗组、西药治疗组,分别于末次感染后10、30、60天处死小鼠,采用HE染色观察心肌病变,采用半定量RTPCR法检测心肌组织中TGFβ1mRNA表达情况。结果HE染色显示各治疗组比模型组心肌病变明显减轻(P<0.05),且TGFβ1mRNA面积较模型组减少(P<0.05)。结论益气降浊汤能通过下调TGFβ1mRNA表达,抑制心肌间质细胞外基质(ECM)的增生和重建,改善心脏病理变化,防止心肌炎后心室重构及向扩张型心肌病转化。Objective To investigate the effects of Yiqi Jiangzhuo Decoction （YJD） on transforming growth factor-β1（TGF-β1） mRNA expression in treating ventricular remodeling in viral myocarditis. Methods After being established into chronic viral myocarditis model by 4 times repetitively infecting with gradient multiplicative CVB3m, 160 male mice were divided into the blank group, the model group, the TCM treated group and the Western medicine treated group. On the 10th, 30th and 60th day after the last time of infection, mice were killed to observe their pathological changes of myocardium with HE staining and to detect TGF-β1 mRNA expression in myocardial tissue with semi-quantitative RT-PCR method. Results Pathological changes of myocardium alleviated, and TGF-β1 mRNA expression distribution area reduced significantly in the two treated groups, as compared with those in the model group （all P〈0.05）. Conclusion YJD could inhibit the hyperplasia and reconstruction of ECM by down-regulating TGF-β1 mRNA expression to improve cardiac pathological changes in myocarditis, so as to prevent the occurrence of ventricular remodeling and the conversion of disease to dilative cardiomyopathy.

关 键 词：益气降浊汤 病毒性心肌炎 转化生长因子Β1 

分 类 号：R285[医药卫生—中药学]