HBV DNA与HBV-m对比分析  

Comparative Analysis between HBV DNA by FQ-PCR and HBV-m by ELISA

作　　者：刘金禄[1] 李采青[1] 卢成[1] 赵海春[1] 

机构地区：[1]河北北方学院附属第一医院微生物科,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2006年第1期24-25,共2页Journal of Hebei North University:Medical Edition

摘　　要：目的:探讨荧光定量PCR检测HBV DNA与HBVm的关系。方法:采用荧光定量PCR法和酶联免疫吸附试验(ELISA)对159份HBVm 8种不同阳性模式及67份HBVm全阴模式血清进行HBV DNA检测。结果:HBsAg、HBcAb、HBeAe阳性者HBV DNA阳性率为89.83%;HBsAg、HBcAb、HBeAb阳性者HBV DNA阳性率为28.81%;HBsAb、HBcAb、HBeAb阳性者HBV DNA阳性率为16.66%;HBVm全阴性者HBV DNA阳性率为17.91%。结论:荧光定量PCR法检测HBV DNA是乙型肝炎病毒复制最直接可信的指标,可反映HBV真实感染和复制状态,特别是低复制状态,明显优于ELISA法检测HBVm,具有重要临床意义。Objective： To investigate the relationship between HBV-DNA determined by the polymerase chain reaction （PCR） assay and Hepatitis-B markers by ELISA method. Methods： The serum samples from 159 cases with 8 different positive models and 67 cases with all negative models were tested by Fluorescence quantitative PCR assay （FQ-PCR） and also by ELISA to compare the results. Results： Among the patients,the positive rate of HBV-DNA for the patients with positive HBsAg, HBcAB and HBeAe was 89.83%;the positive rate of HBV-DNA for the patients with positive HBsAg, HBcAb and HBsAb was 28.81% ; the positive rate of HBV-DNA for the patients with positive HBsAb, HBcAb and HBeAb was 16.66 % ;the positive rate of H BV DNA for the patients with all negative H BVm was 17.91%. Conclusione： H BV-DNA determined by PCR assay is much more corrct and sensitive than HBV-markers by ELISA assay. FQ-PCR could be used as a excellent monitor for the state of HBV irfection and its complication, especially for the state of low complication.

关 键 词：乙型肝炎病毒 脱氧核糖核酸 荧光定量聚合酶链反应 血清标志物 酶联免疫吸附试验 

分 类 号：R446.1[医药卫生—诊断学]