平律复方对大鼠心肌缺血-再灌注心律失常的影响  

Effect of Pinglü Formula on arrhythmia of myocardial ischemia-reperfusion in rats

作　　者：安薇[1] 杨静[1] 张建忠 汪俊军 刘茂南 

机构地区：[1]武汉大学医学院药理学系,湖北武汉430071 [2]武汉市新洲区人民医院,湖北武汉430400

出　　处：《中草药》2006年第4期565-568,共4页Chinese Traditional and Herbal Drugs

基　　金：湖北省武汉市科技局科学技术计划项目(Z20036002009)

摘　　要：目的研究平律复方对大鼠心肌缺血-再灌注心律失常的影响。方法采用结扎冠状动脉左前降支15m in,再灌注45 m in造成大鼠心肌缺血-再灌注模型,监测标准Ⅱ导联心电图变化,测定心肌细胞膜C a2+-ATP酶、M g2+-ATP酶及N a+,K+-ATP酶的活力,采用RP-HPLC法检测心肌组织ATP水平、腺苷酸池(TAN)水平和能量负荷值(EC)。结果平律复方(ig,0.04、0.20、1.00 g/kg)能有效降低大鼠心肌缺血-再灌注心律失常的发生率,显著缩短室性心律失常的持续时间;能对抗缺血-再灌注心肌ATP酶活力的异常变化;各剂量组心肌组织ATP水平恢复为正常对照组的58%、68%和86%,TAN水平和EC值比模型对照组分别增加了25%、49%、66%和24%、27%、29%。结论平律复方具有抗大鼠心肌缺血-再灌注心律失常的作用,其机制可能与改善心肌组织的能量代谢有关。Objective To study the effect of Pinglü Formula （PL） on the arrhythmia of myocardial ischemia-reperfusion in rats. Methods Rat arrhythmia models were established by occlusion of the LAD coronary artery for 15 min and thereafter reperfusion for 45 min. At the same time, the changes were observed on lead Ⅱ dynamic ECG. The activities of Ca^2＋-ATPase, Mg^2＋-ATPase, and Na^＋, K^＋-ATPase were determined, respectively. The levels of ATP, total adenine nucleotides （TAN）, and energy charge （EC） of the myocardial organized energy state were analyzed by RP-HPLC. Results PL （ig 0. 04, 0. 20, and 1.00 g/kg） showed to decrease the incidence of arrhythmia induced by ischemia-reperfusion, shorten the duration of ventricular arrhythmia and eliminate the incidence of ventricular fibrillation. The activities of Ca^2＋-ATPase, Mg^2＋-ATPase, and Na^＋, K^＋-ATPase were increased by PL. In the PL （0. 04, 0. 20, and 1.00 g/kg） groups, ATP levels recovered to 58%, 68%, and 86% as compared with normal group, simul-taneously, TAN values were enhanced to 25%, 49%, and 66% and EC values to 24%, 27%,and 29%, respectively. Conclusion PL has obvious antiarrhythmic effect on rat, the protective action is related to the improvement of energy metabolism.

关 键 词：平律复方 心肌缺血-再灌注 心律失常 能量代谢 

分 类 号：R285.5[医药卫生—中药学]