利用RAPD分析13种石斛属植物的遗传多样性和亲缘关系  

Analysis of genetic diversity and affinity relationships among 13 species of Dendrobium Sw.by RAPD

作　　者：王慧中[1] 卢江杰[1] 施农农[1] 应奇才[1] 

机构地区：[1]杭州师范学院生物化学与分子生物学杭州市重点实验室,浙江杭州310036

出　　处：《中草药》2006年第4期588-592,共5页Chinese Traditional and Herbal Drugs

基　　金：浙江省自然科学基金(301406);浙江省"151"和杭州市"131"人才基金资助项目

摘　　要：目的通过对13种石斛属植物进行遗传多样性和亲缘关系分析,为更好地开发利用石斛资源奠定基础。方法随机扩增多态性DNA(RAPD)技术分析遗传多样性,U PGM A类平均法进行聚类分析。结果利用筛选出的10个随机引物对供试材料DNA进行随机扩增,共得到188条带,其中多态性带有180条,多态性百分率为95.74%。采用U PGM A类平均法对扩增出的谱带进行遗传聚类分析,得出反映各种间亲缘关系的树状图。在遗传距离(D)=0.63处,可将供试材料分为3类,RAPD对基因组的分析结果与传统分类学的结果差异不大。结论该标记技术对石斛属植物的遗传多样性和分类研究是可行的。Objective Genetic diversity and affinity relationships among 13 species of Dendrobium Sw. were analyzed, the result laid a solid foundation for the better use of this resources. Methods Random amplified polymorphic DNA （RAPD） technique was used to analyze genetic diversity, and the dendrogram was constructed by UPGMA. Results Ten RAPD primers were applied to do random amplification. A total of 188 DNA bands was detected, 180 among which were polymorphic, the average rate of polymorphic bands was 95.74%. The result of cluster analysis by using UPGMA method showed that 13 genotypes could be classified into three types in genetic distance 0. 63. This outcome was corresponding to the result by using traditional classification. Conclusion It is concluded that RAPD markers can be used on the studies of genetic relationships and classification of species of Dendrobium Sw. sensitively.

关 键 词：石斛属 RAPD 遗传多样性 亲缘关系 

分 类 号：R282.7[医药卫生—中药学]