T淋巴细胞核仁形成区嗜银蛋白在不同人群中表达含量的探讨  

Expression of T lymphocytes argyrophilic nucleolar organizer region protein in different subgroups

作　　者：王玉萍[1] 费书颖[2] 马亮[3] 钟明华[3] 廖军鲜[3] 

机构地区：[1]中日友好医院妇产科,北京100029 [2]中日友好医院检验科,北京100029 [3]中日友好医院血液科,北京100029

出　　处：《中日友好医院学报》2006年第2期79-81,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的:探讨T淋巴细胞核仁形成区嗜银蛋白(Ag-NORs)在新生儿、正常人和恶性肿瘤患者中免疫功能评价的意义。方法:对培养后的新生儿、恶性肿瘤患者及正常成年人的淋巴细胞进行普通细胞银染色,用KL型肿瘤免疫图像分析仪检测T淋巴细胞核仁银染面积与细胞核面积比值(I.S%)。结果:新生儿的T淋巴细胞Ag-NORs测定值是7.79±0.57%,正常成年人为7.56±0.51%,恶性肿瘤患者为5.15±0.68%。恶性肿瘤患者的T淋巴细胞Ag-NORs较正常人及新生儿均显著降低(均P<0.01),新生儿与正常人相比无显著性差异。结论:恶性肿瘤患者T淋巴细胞的Ag-NORs测定值较低,可能与免疫系统受损有关。Objective：To determine the T lymphocyte argyrophilic nucleolar organizer region protein（Ag-NORs） in infants ,normal adults and malignant tumor patients to evaluate the indicator of different groups of immune state.Methods：A KL tumor immune image analysis system was used to analyze the areas of Ag-NORs in T cells,T cells from peripheral and cod artery blood which were cultivated stimulated,and silver stained.The areas of Ag-NORs were analyzed.Results：The value of infant＇s Ag-NORs was 7.79±0：57%;the value of normal adult was 7.56±0.51% and the value of malignant tumor group was 5.15±0.68%.The T lymphoeytes＇ Ag- NORs of T lymphocytes＇ Ag-NORs was lower than infant and normal adult（P〈0.01）.There was no obvious difference in T lymphoeytes＇ Ag-NORs between infant and normal adult.Conclusion：The T lympboeytes＇ Ag- NORs of malignant tumor group is low,maybe it is associated with the destroyed immune system.

关 键 词：核仁形成区嗜银蛋白 T淋巴细胞 新生儿 恶性肿瘤 

分 类 号：R446.6[医药卫生—诊断学]