BMP-2和EGF对神经干细胞向神经元分化及Notch信号分子表达的影响  

EFFECTS OF BMP-2 AND EGF ON NEURONAL DIFFERENTIATION OF NEURAL STEM CELLS AND EXPRESSION OF NOTCH SIGNAL MOLECULES

作　　者：张琦[1] 林玲[1] 胡建石[1] 郑志竑[1] 

机构地区：[1]福建医科大学分子医学研究中心,福州350004

出　　处：《神经解剖学杂志》2006年第2期158-162,共5页Chinese Journal of Neuroanatomy

基　　金：福建省科技计划重大项目(2001Z020);福建省科技计划(2005K044)资助项目

摘　　要：采用BMP-2(bonemorphogeneticprotein2)和EGF(epidemicgrowthfactor)诱导体外培养的神经干细胞分化,通过免疫荧光染色以及细胞计数方法,观察其分化为神经元的情况;用RTPCR和Westernblot检测Notch信号分子Notch1、PS1、RBPJk和HES1的表达水平。结果表明:BMP2组分化为神经元的比例明显高于对照(control,Ctrl)组和EGF组;EGF组分化为神经元的比例与Ctrl组相比无显著差别;与Ctrl组相比,BMP-2组和EGF组中Notch通路4种信号分子的mRNA表达量均无显著差异;PS1蛋白表达量也无明显差异。结果提示,BMP-2对神经干细胞分化为神经元的诱导作用强于EGF和自主分化,其分化为神经元的比例增加与Notch信号分子的表达水平无关。Neural stem cells were induced to differentiate by BMP-2 （ bone morphogenetic protein 2 ） and EGF （ epidemic growth factor） in vitro respectively. Neuronal differentiation of neural stem cells was identified by immunofluorescence staining and cell counting, RT-PCR and Western-blot were employed to assay the expression of Notch pathway signal molecules： Notchl, PSI, RBP-Jk and HESI. The results revealed that the percentage of neurons in BMP-2 group was much higher as compared with EGF and Ctrl （control） groups; The percentage of neurons showed no difference between EGF and Ctrl groups. Neither the mRNA expression of Notchl, PSI, RBP-Jk and HESI nor the expression of PSI protein showed any difference among three groups. The present results indicate that BMP-2 shows more influence on neuronal differentiation of neural stem cells than EGF and Ctrl and there is no relationship between the increase of neurons induced with BMP-2 and Notch signaling.

关 键 词：神经干细胞 神经元 NOTCH信号通路 BMP-2 EGF 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]