分光光度法测定脉康合剂中木耳多糖含量  

Determination of Agaric Polysaccharide in Maikang Mixture by Visible Spectrophotography

作　　者：田宝林[1] 

机构地区：[1]河北省药品检验所,河北石家庄050011

出　　处：《中国药业》2006年第5期51-52,共2页China Pharmaceuticals

摘　　要：目的研究脉康合剂中木耳多糖的含量测定方法。方法采用分光光度法,以5％苯酚溶液与硫酸显色,测定波长为490 nm。结果 D-无水葡萄糖浓度线性范围是2．46-7．38μg/mL,r=0．999 5;平均回收率为99．73％,RSD=1．11％。结论该方法简便,结果准确,重现性好,可作为脉康合剂的含量测定方法。Objective To study the determination of agaric polysaccharide in Maikang mixture by visible spectrophotography. Methods Adopting the 5% phenol and vitriol as color indicator, agaric polysaccharide was measured by visible spectrophotography. The detective wavelength was 490 nm. Results The method was linear in the range of 2.46- 7.38μg of anhydrous glucose. The average recovery, was 99.73%, RSD was 1. 11%. Conclusion The method is sensitive, accurate and repeatable. The established standard is testified as an effective quality control method for Maikang mixture.

关 键 词：脉康合剂 木耳多糖 含量测定 分光光度法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]