用聚合酶链反应检测多形红斑皮损中单纯疱疹病毒的DNA  

Identification of Herpes Simplex Virus DNA in Lesions of Erythema Multiforme by Polymerase Chain Reaction

作　　者：吴安[1] 陈佳[1] 李冠群[1] 朱学骏[1] 

机构地区：[1]西安医科大学第二临床医学院皮肤科,北京医科大学第一医院皮肤科

出　　处：《中国皮肤性病学杂志》1996年第3期135-136,共2页The Chinese Journal of Dermatovenereology

摘　　要：用聚合酶链反应检测２３例多形红斑石蜡组织切块中ＨＳＶ－ＤＮＡ，１６例为阳性（６９．５６％），其中ＨＳＶ－Ⅰ型１３例（８１．２５％），ＨＳＶ－Ⅱ型３例（１８．７５％）。对照组为银屑病３例，大疱性类天疱疮５例，扁平苔藓３例均为阴性。对于探讨ＨＳＶ在多形红斑发病机理中的作用有重要意义。In this paper formalin-fixed and paraffin-embedded skin biopsies of lesions from 23 patients with erythema multiforme(EM)and 11 controls were detected for the presence of herpes simplex virus(HSV) dy polymerase chain reaction(PCR)staining with haematoxylin and eosin. HSV-specific DNAwas deteced in 16(69.56% )patients.HSV-Ⅰin 13(81.25 %)patients;the other three were HSV-Ⅱ.The controls were all negative(include psoriasis,bullous pemphigoid,lichen planus).There was no significant difference in pathology between HSV-relatedand HSV-negative cases of EM.Our results support the association of HSVin the pathogenesis of some cases of EM.Finally,PCRtechnique provides a quick and effective method of detecting HSV in lesions of herpes-associated EM.

关 键 词：聚合酶链反应 单纯疱疹病毒 DNA 多形红斑 

分 类 号：R758.610.4[医药卫生—皮肤病学与性病学]